{"doi":"10.1101/2020.06.25.169177","title":"Engineering degradation-resistant messenger RNAs in yeast","abstract":"ABSTRACT Virally-derived RNA structures provide useful tools for engineering messenger RNA (mRNA) transcripts with unique properties. Here we show that 5’→3’ e x oribonuclease- r esistant RNA structures (xrRNAs) from Flaviviruses can be used to protect heterologously-expressed messenger RNAs (mRNAs) from 5’→3’ degradation in Saccharomyces cerevisiae (budding yeast). Installation of xrRNAs ahead of a downstream internal ribosome entry site (IRES) leads to the accumulation of partially-degraded mRNAs designed to undergo cap-independent translation. In a yeast strain favoring cap-independent translation, this strategy led to a 30-fold increase in the enzymatic activity of lysates obtained from cells expressing degradation-resistant reporters. Overall, these finding demonstrate the possibility of coupling multiple viral RNA structures together to enhance protein expression.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":125107,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":407150,"name":"Andrea MacFadden","orcid":"0000-0002-5960-6109","position":1,"is_corresponding":false},{"id":16335,"name":"Jeffrey Scott Kieft","orcid":"0000-0002-3718-1891","position":2,"is_corresponding":false},{"id":87044,"name":"Jay R. Hesselberth","orcid":"0000-0002-6299-179X","position":3,"is_corresponding":false},{"id":570795,"name":"Erich G. Chapman","orcid":"0000-0002-5321-9095","position":4,"is_corresponding":false},{"id":571755,"name":"Ana Luisa Franklin","orcid":null,"position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-18T23:15:15.482227Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}