{"doi":"10.1101/2020.06.23.166439","title":"Spinal Column Architecture of the Flexible SPP1 Bacteriophage Tail Tube","abstract":"Abstract Phage therapy has recently regained attention at combating multidrug-resistant bacteria. In 2019, tailed bacteriophages of the Siphoviridae family were engineered to successfully treat a disseminated bacterial infection after all other drugs had failed.( 1 ) This family of phages features a long, flexible, non-contractile tail that has been difficult to characterize structurally. Here, we present the atomic structure of the tail-tube of the bacteriophage SPP1 – a member of this family. Our hybrid structure is based on the integration of structural restraints from solid-state NMR and a density map from cryo-EM. We show that the tail tube protein (TTP) gp17.1 organizes into hexameric rings that are stacked by flexible linker domains and, thus, form a hollow flexible tube with a negatively charged lumen suitable for the transport of DNA. One sentence summary Integrative structural biology by solid-state NMR and cryo-EM enables structure determination of the flexible tail of the bacteriophage SPP1.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126935,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9531,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":317942,"name":"K. Sachowsky","orcid":"0000-0002-5154-5217","position":1,"is_corresponding":false},{"id":317943,"name":"Carl Öster","orcid":"0000-0002-8723-4533","position":2,"is_corresponding":false},{"id":317944,"name":"Sophie Zinn‐Justin","orcid":"0000-0002-7680-1088","position":3,"is_corresponding":false},{"id":317945,"name":"Raimond B. G. Ravelli","orcid":"0000-0001-6056-5888","position":4,"is_corresponding":false},{"id":317946,"name":"Gunnar F. Schröder","orcid":"0000-0003-1803-5431","position":5,"is_corresponding":false},{"id":317947,"name":"Michael Habeck","orcid":"0000-0002-2188-5667","position":6,"is_corresponding":false},{"id":317948,"name":"Adam Lange","orcid":"0000-0002-7534-5973","position":7,"is_corresponding":false},{"id":317941,"name":"Maximilian Zinke","orcid":"0000-0002-0541-5139","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}