{"doi":"10.1101/2020.06.21.163469","title":"Vegetative phase change in <i>Populus tremula x alba</i>","abstract":"Summary Plants transition through juvenile and adult phases of vegetative development in a process known as vegetative phase change (VPC). In poplars (genus Populus ) the differences between these stages are subtle, making it difficult to determine when this transition occurs. Previous studies of VPC in poplars have relied on plants propagated in vitro , leaving the natural progression of this process unknown. We examined developmental morphology of seed-grown and in vitro derived Populus tremula x alba (clone 717-1B4), and compared the phenotype of these, to transgenics with manipulated miR156 expression, the master regulator of VPC. In seed-grown plants, most traits changed from node-to-node during the first 3 months of development but remained constant after node 25. Many traits remained unchanged in clones over-expressing miR156, or were enhanced when miR156 was lowered, demonstrating their natural progression is regulated by the miR156/SPL pathway. The characteristic leaf fluttering of Populus is one of these miR156-regulated traits. Vegetative development in plants grown from culture mirrored that of seed-grown plants, allowing direct comparison between plants often used in research and those found in nature. These results provide a foundation for further research on the role of VPC in the ecology and evolution of this economically important genus.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126952,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9606,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":575007,"name":"Aaron R. Leichty","orcid":"0000-0003-1071-7921","position":1,"is_corresponding":false},{"id":575008,"name":"Erin Doody","orcid":"0000-0002-1282-1792","position":2,"is_corresponding":false},{"id":575009,"name":"Cathleen Ma","orcid":"0000-0002-5387-4719","position":3,"is_corresponding":false},{"id":575010,"name":"Steven H. Strauss","orcid":"0000-0001-9670-3082","position":4,"is_corresponding":false},{"id":407334,"name":"R. Scott Poethig","orcid":"0000-0001-6592-5862","position":5,"is_corresponding":false},{"id":575006,"name":"Erica H. Lawrence","orcid":"0000-0002-0220-3210","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}