{"doi":"10.1101/2020.06.18.158576","title":"Reconstitution of an intact clock that generates circadian DNA binding in vitro","abstract":"Abstract Circadian clocks control gene expression in the complex milieu of cells. Here, we reconstituted under defined conditions in vitro the cyanobacterial circadian clock system which includes an oscillator, signal-transduction pathways, transcription factor, and promoter DNA. The system oscillates autonomously with a near 24 h period, remains phase coherent for many days, and allows real-time observation of each component simultaneously without user intervention. This reassembled clock system provides new insights into how a circadian clock exerts control over gene expression and can serve in the area of synthetic biology as a new platform upon which to build even more complexity. One Sentence Summary An autonomously oscillating circadian clock-controlled gene regulatory circuit is studied in vitro using a real-time high-throughput assay.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":125078,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9429,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":564740,"name":"Dustin C. Ernst","orcid":"0000-0001-8762-960X","position":1,"is_corresponding":false},{"id":570743,"name":"Mingxu Fang","orcid":"0000-0003-1595-8046","position":2,"is_corresponding":false},{"id":564739,"name":"Cigdem Sancar","orcid":"0000-0002-7242-8757","position":3,"is_corresponding":false},{"id":285740,"name":"Carrie L. Partch","orcid":"0000-0002-4677-2861","position":4,"is_corresponding":false},{"id":272270,"name":"Susan S. Golden","orcid":"0000-0002-4264-7019","position":5,"is_corresponding":false},{"id":378220,"name":"Andy LiWang","orcid":"0000-0003-4741-6946","position":6,"is_corresponding":false},{"id":378218,"name":"Archana G. Chavan","orcid":"0000-0002-5691-8094","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:15:15.482227Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}