{"doi":"10.1101/2020.06.08.135129","title":"Single-cell RNA transcriptomics identifies Hivep3 as essential in regulating the development of innate-like T lymphocytes","abstract":"Summary Most T lymphocytes leave the thymus as naïve cells with limited functionality. However, unique populations of T cells, commonly known as innate-like T cells, differentiate into functionally distinct effector subsets during thymic development under the influence of the transcription factor PLZF. Here, we profiled >10,000 differentiating thymic iNKT cells using single-cell RNA sequencing to provide a comprehensive transcriptional landscape of their maturation, function, and fate decision in steady state. We identified Hivep3 , a zinc finger transcription factor and adaptor protein, as a key factor that is expressed in early precursors and regulates the post-selection proliferative burst, differentiation and functions of iNKT cells. Importantly, we extended these results to other PLZF + innate-like T cell populations, highlighting the unique and common requirement of Hivep3 to the development of all innate-like T cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":125037,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9519,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":300977,"name":"Jingjing Zhang","orcid":"0000-0002-7953-1159","position":1,"is_corresponding":false},{"id":303089,"name":"Mary Jessamine Michaels-Foster","orcid":null,"position":2,"is_corresponding":false},{"id":11285,"name":"Tonya M. Brunetti","orcid":"0000-0002-2228-2198","position":3,"is_corresponding":false},{"id":300978,"name":"Liyen Loh","orcid":"0000-0002-1676-0579","position":4,"is_corresponding":false},{"id":300979,"name":"James Scott‐Browne","orcid":"0000-0002-0546-7799","position":5,"is_corresponding":false},{"id":300980,"name":"Laurent Gapin","orcid":"0000-0001-9730-5174","position":6,"is_corresponding":false},{"id":570684,"name":"Sai Harsha Krovi","orcid":null,"position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-18T23:15:11.632153Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}