{"doi":"10.1101/2020.06.05.136192","title":"A Genome-wide Library of MADM Mice for Single-Cell Genetic Mosaic Analysis","abstract":"SUMMARY Mosaic Analysis with Double Markers (MADM) offers a unique approach to visualize and concomitantly manipulate genetically-defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage; single-cell morphology and physiology; genomic imprinting phenotypes; and dissection of cell-autonomous gene functions in vivo in health and disease. Yet, MADM could only be applied to <25% of all mouse genes on select chromosomes thus far. To overcome this limitation, we generated transgenic mice with knocked-in MADM cassettes near the centromeres of all 19 autosomes and validated their use across organs. With this resource, >96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond proof-of-principle, we applied our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We found striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":119906,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8042,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":556075,"name":"Amarbayasgalan Davaatseren","orcid":"0000-0002-4022-9542","position":1,"is_corresponding":false},{"id":556076,"name":"Nicole Amberg","orcid":"0000-0002-3183-8207","position":2,"is_corresponding":false},{"id":556743,"name":"Andi H. Hansen","orcid":null,"position":3,"is_corresponding":false},{"id":556077,"name":"Johanna Sonntag","orcid":"0000-0002-5066-808X","position":4,"is_corresponding":false},{"id":556744,"name":"Lill Andersen","orcid":null,"position":5,"is_corresponding":false},{"id":556745,"name":"Tina Bernthaler","orcid":null,"position":6,"is_corresponding":false},{"id":556078,"name":"Anna Heger","orcid":"0009-0005-9662-3118","position":7,"is_corresponding":false},{"id":246168,"name":"Randy L. Johnson","orcid":"0000-0003-4549-3593","position":8,"is_corresponding":false},{"id":199261,"name":"Lindsay A. Schwarz","orcid":null,"position":9,"is_corresponding":false},{"id":242246,"name":"Liqun Luo","orcid":"0000-0001-5467-9264","position":10,"is_corresponding":false},{"id":325915,"name":"Thomas Rülicke","orcid":"0000-0002-2121-9496","position":11,"is_corresponding":false},{"id":325917,"name":"Simon Hippenmeyer","orcid":"0000-0003-2279-1061","position":12,"is_corresponding":false},{"id":325916,"name":"Ximena Contreras","orcid":"0000-0002-5606-8739","position":0,"is_corresponding":true}],"reference_count":104,"raw_metadata":null,"created_at":"2026-07-18T23:14:13.002105Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}