{"doi":"10.1101/2020.06.04.134510","title":"T cell receptor is required for differentiation but not maintenance of intestinal intraepithelial lymphocytes","abstract":"Summary The gut epithelium is populated by intraepithelial lymphocytes (IELs), a heterogeneous T cell population with cytotoxic and regulatory properties. Migrating peripheral CD4 + T cells, including regulatory (Treg) and conventional T cells (Tconv), acquire an IEL (CD4-IEL) program upon arrival at the epithelium. However, the specific role of the T cell receptor (TCR) in this process remains unclear. Single-cell TCR repertoire and transcriptomic analysis of intraepithelial CD4 + T cells revealed different extents of clonal expansion and TCR overlap between cell states; fully differentiated CD4-IELs from Tregs or Tconvs were the least diverse. Conditional deletion of TCR on differentiating CD4 + T cells or of MHCII on intestinal epithelial cells prevented CD4-IEL differentiation. However, TCR ablation on developed CD4-IELs did not affect their accumulation. These results indicate that local recognition of a limited set of antigens is an essential signal for the differentiation and adaptation of T cells to the epithelium.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126880,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9497,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":295161,"name":"Mariya London","orcid":"0000-0002-9712-0070","position":1,"is_corresponding":false},{"id":217900,"name":"Tiago B. R. Castro","orcid":"0000-0002-0359-0611","position":2,"is_corresponding":false},{"id":295162,"name":"Luka Mesin","orcid":"0000-0001-9852-6841","position":3,"is_corresponding":false},{"id":296758,"name":"Suppawat Kongthong","orcid":null,"position":4,"is_corresponding":false},{"id":295164,"name":"Audrey Harnagel","orcid":"0000-0002-8243-9230","position":5,"is_corresponding":false},{"id":266895,"name":"Gabriel D. Victora","orcid":"0000-0001-8807-348X","position":6,"is_corresponding":false},{"id":66190,"name":"Daniel Mucida","orcid":"0000-0002-0000-0452","position":7,"is_corresponding":false},{"id":295160,"name":"Angelina M. Bilate","orcid":"0000-0003-4575-786X","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}