{"doi":"10.1101/2020.06.02.130690","title":"A Rab Escort Protein Regulates the MAPK Pathway That Controls Filamentous Growth in Yeast","abstract":"ABSTRACT MAPK pathways regulate different responses yet can share a subset of common components. In this study, a genome-wide screen was performed to identify genes that, when overexpressed, induce a growth reporter ( FUS1-HIS3 ) that responds to ERK-type MAPK pathways (Mating/filamentous growth or fMAPK) but not p38-type MAPK pathways (HOG) in yeast. Approximately 4,500 plasmids overexpressing individual yeast genes were introduced into strains containing the FUS1-HIS3 reporter by high-throughput transformation. Candidate genes were identified by measuring the degree of growth, which was a reflection of reporter activity. Of fourteen genes identified and validated by re-testing, two were metabolic controls ( HIS3 and ATR1 ), five had established roles in regulating ERK-type pathways ( STE4, STE7, BMH1, BMH2, MIG2 ) and seven represent potentially new regulators of MAPK signaling ( RRN6, CIN5, MRS6, KAR2, TFA1, RSC3, RGT2 ). MRS6 encodes a Rab escort protein and effector of the TOR pathway that plays an established role in nutrient signaling. MRS6 overexpression stimulated filamentous/invasive growth and phosphorylation of the ERK-type fMAPK, Kss1. Overexpression of MRS6 reduced the osmotolerance of cells and phosphorylation of the p38/HOG pathway MAPK, Hog1. Mrs6 interacted with the PAK kinase Ste20 and MAPKK Ste7 by two-hybrid analysis. Collectively, the data indicate that Mrs6 may function to selectively propagate an ERK-dependent signal. Generally speaking, the identification of new MAPK pathway regulators by genetic screening in yeast may be a useful resource for understanding signaling pathway regulation.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126892,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9565,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":392449,"name":"Paul J. Cullen","orcid":"0000-0002-6703-1480","position":1,"is_corresponding":false},{"id":425552,"name":"Sheida Jamalzadeh","orcid":null,"position":0,"is_corresponding":true}],"reference_count":133,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}