{"doi":"10.1101/2020.05.28.121640","title":"Single-dose replicating RNA vaccine induces neutralizing antibodies against SARS-CoV-2 in nonhuman primates","abstract":"The ongoing COVID-19 pandemic, caused by infection with SARS-CoV-2, is having a dramatic and deleterious impact on health services and the global economy. Grim public health statistics highlight the need for vaccines that can rapidly confer protection after a single dose and be manufactured using components suitable for scale-up and efficient distribution. In response, we have rapidly developed repRNA-CoV2S, a stable and highly immunogenic vaccine candidate comprised of an RNA replicon formulated with a novel Lipid InOrganic Nanoparticle (LION) designed to enhance vaccine stability, delivery and immunogenicity. We show that intramuscular injection of LION/repRNA-CoV2S elicits robust anti-SARS-CoV-2 spike protein IgG antibody isotypes indicative of a Type 1 T helper response as well as potent T cell responses in mice. Importantly, a single-dose administration in nonhuman primates elicited antibody responses that potently neutralized SARS-CoV-2. These data support further development of LION/repRNA-CoV2S as a vaccine candidate for prophylactic protection from SARS-CoV-2 infection.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":118933,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":238499,"name":"Amit P. Khandhar","orcid":"0000-0003-4049-1855","position":1,"is_corresponding":false},{"id":97046,"name":"Alexandra C. Walls","orcid":"0000-0002-9636-8330","position":2,"is_corresponding":false},{"id":108904,"name":"Emily A. Hemann","orcid":"0000-0002-6398-3492","position":3,"is_corresponding":false},{"id":225376,"name":"Megan A. O’Connor","orcid":"0000-0003-2127-5073","position":4,"is_corresponding":false},{"id":238500,"name":"Patience Murapa","orcid":"0000-0002-5824-9672","position":5,"is_corresponding":false},{"id":239424,"name":"Jacob Archer","orcid":null,"position":6,"is_corresponding":false},{"id":239425,"name":"Shanna Leventhal","orcid":null,"position":7,"is_corresponding":false},{"id":229229,"name":"Jim Fuller","orcid":null,"position":8,"is_corresponding":false},{"id":225388,"name":"Thomas B. Lewis","orcid":"0009-0003-8463-6718","position":9,"is_corresponding":false},{"id":239426,"name":"Kevin E. Draves","orcid":null,"position":10,"is_corresponding":false},{"id":239427,"name":"Samantha Randall","orcid":null,"position":11,"is_corresponding":false},{"id":225384,"name":"Kathryn A. Guerriero","orcid":"0000-0001-9028-7102","position":12,"is_corresponding":false},{"id":238502,"name":"Malcolm S. Duthie","orcid":"0000-0002-8515-5516","position":13,"is_corresponding":false},{"id":238503,"name":"Darrick Carter","orcid":"0000-0001-5901-3402","position":14,"is_corresponding":false},{"id":225987,"name":"Steven G. Reed","orcid":"0000-0003-2193-100X","position":15,"is_corresponding":false},{"id":238504,"name":"David W. Hawman","orcid":"0000-0001-8233-8176","position":16,"is_corresponding":false},{"id":107426,"name":"Heinz Feldmann","orcid":"0000-0001-9448-8227","position":17,"is_corresponding":false},{"id":108916,"name":"Michael Gale","orcid":"0000-0002-6332-7436","position":18,"is_corresponding":false},{"id":97051,"name":"David Veesler","orcid":"0000-0002-6019-8675","position":19,"is_corresponding":false},{"id":238505,"name":"Peter Berglund","orcid":"0000-0003-2367-1686","position":20,"is_corresponding":false},{"id":225390,"name":"Deborah H. Fuller","orcid":"0000-0001-7315-2441","position":21,"is_corresponding":false},{"id":238498,"name":"Jesse H. Erasmus","orcid":"0000-0003-1612-2697","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-18T23:14:03.409507Z","pmid":"32511417","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}