{"doi":"10.1101/2020.05.28.118703","title":"Amnion signals are essential for mesoderm formation in primates","abstract":"Abstract Essential genes for murine embryonic development can demonstrate a disparate phenotype in human cohorts. By generating a transcriptional atlas containing >30,000 cells from postimplantation non-human primate embryos, we discovered that ISL1 , a gene with a well-established role in cardiogenesis, controls a gene regulatory network in primate amnion. CRISPR/Cas9-targeting of ISL1 resulted in non-human primate embryos which did not yield viable offspring, demonstrating that ISL1 is critically required in primate embryogenesis. On a cellular level, mutant ISL1 embryos displayed a failure in mesoderm formation due to reduced BMP4 signaling from the amnion. Via loss of function and rescue studies in human embryonic stem cells we confirmed a similar role of ISL1 in human in vitro derived amnion. This study highlights the importance of the amnion as a signaling center during primate mesoderm formation and demonstrates the potential of in vitro primate model systems to dissect the genetics of early human embryonic development.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":118995,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.955,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":552641,"name":"Alexander Goedel","orcid":"0000-0002-5980-2257","position":1,"is_corresponding":false},{"id":552642,"name":"Yu Kang","orcid":"0000-0003-1730-6498","position":2,"is_corresponding":false},{"id":531421,"name":"Chenyang Si","orcid":"0000-0002-3354-1968","position":3,"is_corresponding":false},{"id":552643,"name":"Chu Chu","orcid":"0000-0002-8151-1157","position":4,"is_corresponding":false},{"id":45506,"name":"Yi Zheng","orcid":"0000-0002-2685-3680","position":5,"is_corresponding":false},{"id":531410,"name":"Zhenzhen Chen","orcid":"0000-0002-8938-1449","position":6,"is_corresponding":false},{"id":379,"name":"Peter J. Gruber","orcid":"0000-0002-7356-905X","position":7,"is_corresponding":false},{"id":457315,"name":"Yao Xiao","orcid":"0000-0001-5654-2042","position":8,"is_corresponding":false},{"id":552644,"name":"Chikai Zhou","orcid":"0000-0001-9653-3466","position":9,"is_corresponding":false},{"id":552645,"name":"Nevin Witman","orcid":"0000-0002-1109-778X","position":10,"is_corresponding":false},{"id":553337,"name":"Chuen-Yan Leung","orcid":null,"position":11,"is_corresponding":false},{"id":531417,"name":"Yongchang Chen","orcid":"0000-0002-3856-3576","position":12,"is_corresponding":false},{"id":45507,"name":"Jianping Fu","orcid":"0000-0001-9629-6739","position":13,"is_corresponding":false},{"id":531413,"name":"Weizhi Ji","orcid":"0000-0003-2550-4224","position":14,"is_corresponding":false},{"id":552646,"name":"Fredrik Lanner","orcid":"0000-0002-2771-7445","position":15,"is_corresponding":false},{"id":531415,"name":"Yuyu Niu","orcid":"0000-0002-8797-4152","position":16,"is_corresponding":false},{"id":552647,"name":"Kenneth R. Chien","orcid":"0000-0002-2759-8378","position":17,"is_corresponding":false},{"id":552640,"name":"Ran Yang","orcid":"0000-0001-7104-4383","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-18T23:14:03.409507Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}