{"doi":"10.1101/2020.05.26.116368","title":"A selective autophagy pathway for phase separated endocytic protein deposits","abstract":"Summary Autophagy eliminates cytoplasmic content selected by autophagy receptors, which link cargoes to the membrane bound autophagosomal ubiquitin-like protein Atg8/LC3. Here, we discover a selective autophagy pathway for protein condensates formed by endocytic proteins. In this pathway, the endocytic yeast protein Ede1 functions as a selective autophagy receptor. Distinct domains within Ede1 bind Atg8 and mediate phase separation into condensates. Both properties are necessary for an Ede1-dependent autophagy pathway for endocytic proteins, which differs from regular endocytosis, does not involve other known selective autophagy receptors, but requires the core autophagy machinery. Cryo-electron tomography of Ede1-containing condensates – at the plasma membrane and in autophagic bodies – shows a phase-separated compartment at the beginning and end of the Ede1-mediated selective autophagy pathway. Our data suggest a model for autophagic degradation of membraneless compartments by the action of intrinsic autophagy receptors. Highlights Ede1 is a selective autophagy receptor for aberrant CME protein assemblies Aberrant CME assemblies form by liquid-liquid phase separation Core autophagy machinery and Ede1 are important for degradation of CME condensates Ultrastrucural view of a LLPS compartment at the PM and within autophagic bodies","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":119795,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9487,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":270905,"name":"Chia‐Wei Lee","orcid":"0000-0002-8925-7421","position":1,"is_corresponding":false},{"id":270906,"name":"Philipp S. Erdmann","orcid":"0000-0001-8289-9133","position":2,"is_corresponding":false},{"id":270907,"name":"Yumei Zheng","orcid":"0000-0002-4033-2670","position":3,"is_corresponding":false},{"id":270909,"name":"Stefan Jentsch","orcid":"0000-0003-4033-9714","position":4,"is_corresponding":false},{"id":270910,"name":"Boris Pfander","orcid":"0000-0003-2180-5054","position":5,"is_corresponding":false},{"id":241650,"name":"Brenda A. Schulman","orcid":"0000-0002-3083-1126","position":6,"is_corresponding":false},{"id":270911,"name":"Wolfgang Baumeister","orcid":"0000-0001-8154-8809","position":7,"is_corresponding":false},{"id":270904,"name":"Florian Wilfling","orcid":"0000-0002-6559-7261","position":0,"is_corresponding":true}],"reference_count":109,"raw_metadata":null,"created_at":"2026-07-18T23:14:13.002105Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}