{"doi":"10.1101/2020.05.23.112250","title":"Transcriptional re-wiring by mutation of the yeast Hsf1 oligomerization domain","abstract":"Abstract Response to heat stress is mediated by heat shock transcription factors (HSFs), which possess conserved DNA-binding and oligomerization domains. The oligomerization domain is required for HSF1 to transition under heat stress from a monomer to a homotrimer, which alters DNA-binding specificity and affinity. Sequence variation in the oligomerization domain affects HSF1 activity, although this link is poorly understood. We performed a deep mutational scan of >400,000 variants of the oligomerization domain of Saccharomyces cerevisiae Hsf1 and measured fitness under stress and non-stress conditions. We identify mutations that confer temperature-specific phenotypes; some exceptional Hsf1variants lead to enhanced growth under heat stress and changes to in vivo DNA-binding and transcriptional programs. The link between Hsf1 oligomerization and DNA-binding domain is evolutionarily conserved, with co-evolving residues between these domains found among fungi. Mutation of transcription factor oligomerization domains may represent a path toward re-wiring transcriptional programs without mutation of DNA-binding domains.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":124928,"datarank":0.18301375371699852,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.018221910416782065,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.018221910416782065,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":239742,"name":"Michael W. Dorrity","orcid":"0000-0002-7691-6504","position":1,"is_corresponding":false},{"id":250225,"name":"Wei Zhou","orcid":"0000-0003-4236-4091","position":2,"is_corresponding":false},{"id":87047,"name":"Stanley Fields","orcid":"0000-0001-5504-5925","position":3,"is_corresponding":false},{"id":239744,"name":"Christine Queitsch","orcid":"0000-0002-0905-4705","position":4,"is_corresponding":false},{"id":570190,"name":"Elizabeth A. Morton","orcid":"0000-0003-3451-4950","position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:15:11.632153Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}