{"doi":"10.1101/2020.05.22.111286","title":"The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes","abstract":"Abstract Slow-wave sleep, defined by low frequency (<4 Hz) electrical brain activity, is a basic brain function affecting metabolite clearance and memory consolidation. Although the origin of low-frequency activity is related to cortical up and down states, the underlying cellular mechanism of how low-frequency activity becomes effective has remained elusive. We applied electrical stimulation to cultured glial astrocytes while monitored the trafficking of GFP-tagged intracellular vesicles using TIRFM. We found a frequency-dependent effect of electrical stimulation that electrical stimulation in low frequency elevates the mobility of astrocytic intracellular vesicles. We suggest a novel mechanism of brain modulation that electrical signals in the lower range frequencies embedded in brainwaves modulate the functionality of astrocytes for brain homeostasis and memory consolidation. This finding suggests a physiological mechanism whereby endogenous low-frequency brain oscillations enhance astrocytic function that may underlie some of the benefits of slow-wave sleep and highlights possible medical device approach for treating neurological diseases.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126709,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9607,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":574598,"name":"Thomas P. Burghardt","orcid":"0000-0003-3119-4074","position":1,"is_corresponding":false},{"id":227179,"name":"Gregory A. Worrell","orcid":"0000-0003-2916-0553","position":2,"is_corresponding":false},{"id":574599,"name":"Hailong Wang","orcid":"0000-0002-6107-5095","position":3,"is_corresponding":false},{"id":15013,"name":"Yi-Hua Wang","orcid":"0000-0003-2045-423X","position":0,"is_corresponding":true}],"reference_count":83,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}