{"doi":"10.1101/2020.05.21.108951","title":"Intrinsic and extrinsic factors collaborate to activate pharyngeal satellite cells without muscle injury","abstract":"Abstract Satellite cells (SCs), adult muscle stem cells in craniofacial muscles proliferate and differentiate/fuse without injury, unlike quiescent SCs in uninjured limb muscle. However, whether intrinsic or extrinsic factors driving their increased basal activity are largely unknown. We compared SCs from the pharynx, which contains constrictor muscles critical for swallowing, to SCs from limb muscle. Pharyngeal SCs are intrinsically more proliferative and contain higher mitochondrial content relative to limb SCs. Pharyngeal SCs occupy less quiescent microenvironments containing collagen V and pharyngeal muscles provide a distinctive SC niche enriched with neighboring resident macrophages and fibroadipogenic progenitors. Loss of SCs impacts pharyngeal myofiber cross-sectional area and the number of neighboring cells, suggesting that SCs are required to maintain pharyngeal muscle homeostasis and its unique niche. Taken together, this study gives new insights to explain the distinctive SC activity of craniofacial muscles, which may explain their unique susceptibility to various muscular dystrophies.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126724,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9581,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":574612,"name":"Yiming Zhang","orcid":"0000-0001-8547-6654","position":1,"is_corresponding":false},{"id":575230,"name":"Wu Fang","orcid":null,"position":2,"is_corresponding":false},{"id":574613,"name":"James P. Allen","orcid":"0000-0002-2124-6881","position":3,"is_corresponding":false},{"id":574614,"name":"Katherine E. Vest","orcid":"0000-0002-3391-8649","position":4,"is_corresponding":false},{"id":296289,"name":"Hyo‐Jung Choo","orcid":"0000-0002-6280-0332","position":5,"is_corresponding":false},{"id":574611,"name":"Eunhye Kim","orcid":"0000-0001-5102-6794","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}