{"doi":"10.1101/2020.05.20.105247","title":"A replication-competent vesicular stomatitis virus for studies of SARS-CoV-2 spike-mediated cell entry and its inhibition","abstract":"There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, and define correlates of immune protection, and to down-select candidate antivirals. Here, we describe a highly infectious recombinant vesicular stomatitis virus bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein that closely resembles the authentic agent in its entry-related properties. We show that the neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S and that neutralization of the rVSV and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific vaccines and therapeutics and for mechanistic studies of viral entry and its inhibition.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":118980,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":109527,"name":"Denise Haslwanter","orcid":"0000-0003-2727-0468","position":1,"is_corresponding":false},{"id":109540,"name":"Robert H. Bortz","orcid":"0000-0003-1751-2152","position":2,"is_corresponding":false},{"id":109541,"name":"Ariel S. Wirchnianski","orcid":"0000-0002-3953-8954","position":3,"is_corresponding":false},{"id":240439,"name":"Gorka Lasso","orcid":"0000-0001-5355-323X","position":4,"is_corresponding":false},{"id":187436,"name":"Olivia Vergnolle","orcid":null,"position":5,"is_corresponding":false},{"id":240440,"name":"Shawn A. Abbasi","orcid":"0000-0002-7516-5876","position":6,"is_corresponding":false},{"id":109544,"name":"J. Maximilian Fels","orcid":"0000-0003-1455-9318","position":7,"is_corresponding":false},{"id":109542,"name":"Ethan Laudermilch","orcid":"0000-0001-7488-1718","position":8,"is_corresponding":false},{"id":109543,"name":"Catalina Florez","orcid":"0000-0002-0407-861X","position":9,"is_corresponding":false},{"id":240441,"name":"Amanda Mengotto","orcid":"0000-0001-6383-422X","position":10,"is_corresponding":false},{"id":243591,"name":"Duncan Kimmel","orcid":null,"position":11,"is_corresponding":false},{"id":240442,"name":"Ryan J. Malonis","orcid":"0000-0002-5759-3180","position":12,"is_corresponding":false},{"id":240443,"name":"George I. Georgiev","orcid":"0000-0002-1323-7600","position":13,"is_corresponding":false},{"id":240444,"name":"Jose A. Quiroz","orcid":"0000-0001-8525-930X","position":14,"is_corresponding":false},{"id":240445,"name":"Jason Barnhill","orcid":"0000-0001-9051-4256","position":15,"is_corresponding":false},{"id":504851,"name":"Liise-anne Pirofski","orcid":"0000-0002-7568-2961","position":16,"is_corresponding":false},{"id":75972,"name":"Johanna P. Daily","orcid":"0000-0003-2506-1072","position":17,"is_corresponding":false},{"id":109549,"name":"John M. Dye","orcid":"0000-0002-0883-5016","position":18,"is_corresponding":false},{"id":240446,"name":"Jonathan R. Lai","orcid":"0000-0002-4863-0015","position":19,"is_corresponding":false},{"id":109525,"name":"Andrew S. Herbert","orcid":"0000-0003-4697-7928","position":20,"is_corresponding":false},{"id":109548,"name":"Kartik Chandran","orcid":"0000-0003-0232-7077","position":21,"is_corresponding":false},{"id":109529,"name":"Rohit K. Jangra","orcid":"0000-0002-3119-0869","position":22,"is_corresponding":false},{"id":109530,"name":"M. Eugenia Dieterle","orcid":"0000-0002-5257-7253","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-18T23:14:03.409507Z","pmid":"32511365","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}