{"doi":"10.1101/2020.05.15.094847","title":"Characterisation of ovine bone marrow-derived stromal cells (oBMSC) and evaluation of chondrogenically induced <i>micro</i> -pellets for cartilage tissue repair <i>in vivo</i>","abstract":"Abstract Background Bone marrow stromal cells (BMSC) show promise in cartilage repair, and sheep are the most common large animal pre-clinical model. The objective of this study was to characterize ovine BMSC (oBMSC) in vitro , and to evaluate the capacity of chondrogenic micro -pellets manufactured from oBMSC or ovine articular chondrocytes (oACh) to repair osteochondral defects in sheep. Methods oBMSC were characterised for surface marker expression using flow cytometry and evaluated for tri-lineage differentiation. oBMSC micro -pellets were manufactured in a microwell platform, and chondrogenesis was compared at 2%, 5%, and 20% O 2 . The capacity of cartilage micro -pellets manufactured from oBMSC or oACh to repair osteochondral defects in adult sheep was evaluated in an 8-week pilot study. Expanded oBMSC were positive for CD44 and CD146 and negative for CD45. Results The common adipogenic induction medium ingredient, 3-Isobutyl-1-methylxanthine (IBMX) was toxic to oBMSC, but adipogenesis could be restored by excluding IBMX from the medium. BMSC chondrogenesis was optimal in a 2% O 2 atmosphere. Micro -pellets formed from oBMSC or oACh appeared morphologically similar, but hypertrophic genes were elevated in oBMSC micro -pellets. While oACh micro -pellets formed cartilage-like repair tissue in sheep, oBMSC micro -pellets did not. Conclusion The sensitivity of oBMSC to IBMX highlights species-species differences between oBMSC and hBMSC. Micro -pellets manufactured from oBMSC were not effective in repairing osteochondral defects, while oACh micro -pellets enabled modest repair. While oBMSC can be driven to form cartilage-like tissue in vitro, their effective use in cartilage repair will require mitigation of hypertrophy.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126678,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":574569,"name":"Ena Music","orcid":"0000-0003-4139-6868","position":1,"is_corresponding":false},{"id":332370,"name":"Pamela Gehron Robey","orcid":"0000-0002-5316-5576","position":2,"is_corresponding":false},{"id":471307,"name":"Stan Gronthos","orcid":"0000-0002-6225-3084","position":3,"is_corresponding":false},{"id":574570,"name":"Ross Crawford","orcid":"0000-0001-6079-1316","position":4,"is_corresponding":false},{"id":574571,"name":"Siamak Saifzadeh","orcid":"0000-0002-0295-7477","position":5,"is_corresponding":false},{"id":399140,"name":"Travis J. Klein","orcid":"0000-0002-6669-7766","position":6,"is_corresponding":false},{"id":5753,"name":"Michael R. Doran","orcid":"0000-0001-5876-4757","position":7,"is_corresponding":false},{"id":574568,"name":"Kathryn Futrega","orcid":"0000-0003-3411-6768","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}