{"doi":"10.1101/2020.05.13.093088","title":"Rapid selection of a human monoclonal antibody that potently neutralizes SARS-CoV-2 in two animal models","abstract":"Effective therapies are urgently needed for the SARS-CoV-2/COVID19 pandemic. We identified panels of fully human monoclonal antibodies (mAbs) from eight large phage-displayed Fab, scFv and VH libraries by panning against the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) glycoprotein. One high affinity mAb, IgG1 ab1, specifically neutralized replication competent SARS-CoV-2 with exceptional potency as measured by two different assays. There was no enhancement of pseudovirus infection in cells expressing Fcγ receptors at any concentration. It competed with human angiotensin-converting enzyme 2 (hACE2) for binding to RBD suggesting a competitive mechanism of virus neutralization. IgG1 ab1 potently neutralized mouse ACE2 adapted SARS-CoV-2 in wild type BALB/c mice and native virus in hACE2 expressing transgenic mice. The ab1 sequence has relatively low number of somatic mutations indicating that ab1-like antibodies could be quickly elicited during natural SARS-CoV-2 infection or by RBD-based vaccines. IgG1 ab1 does not have developability liabilities, and thus has potential for therapy and prophylaxis of SARS-CoV-2 infections. The rapid identification (within 6 days) of potent mAbs shows the value of large antibody libraries for response to public health threats from emerging microbes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":119134,"datarank":0.49983067652628066,"base_score":3.332204510175204,"endowment":3.332204510175204,"self_citation_contribution":0.49983067652628066,"citation_network_contribution":0.0,"self_endowment_contribution":0.49983067652628066,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":255555,"name":"Aleksandra Drelich","orcid":"0000-0002-0201-5017","position":1,"is_corresponding":false},{"id":105498,"name":"David R. Martinez","orcid":"0000-0002-2940-8961","position":2,"is_corresponding":false},{"id":105508,"name":"Lisa E. Gralinski","orcid":"0000-0003-1374-8002","position":3,"is_corresponding":false},{"id":267223,"name":"Chuan Chen","orcid":"0000-0001-5701-0857","position":4,"is_corresponding":false},{"id":267224,"name":"Zehua Sun","orcid":"0000-0003-4287-1763","position":5,"is_corresponding":false},{"id":105112,"name":"Alexandra Schäfer","orcid":null,"position":6,"is_corresponding":false},{"id":105111,"name":"Sarah R. Leist","orcid":"0000-0002-4989-5381","position":7,"is_corresponding":false},{"id":269276,"name":"Xianglei Liu","orcid":null,"position":8,"is_corresponding":false},{"id":288080,"name":"Doncho V. Zhelev","orcid":"0000-0001-8800-7044","position":9,"is_corresponding":false},{"id":15029,"name":"Liyong Zhang","orcid":"0000-0002-6952-7463","position":10,"is_corresponding":false},{"id":88038,"name":"Eric C. Peterson","orcid":"0000-0002-9466-3272","position":11,"is_corresponding":false},{"id":288083,"name":"Alex Conard","orcid":"0000-0003-0856-134X","position":12,"is_corresponding":false},{"id":258969,"name":"John W. Mellors","orcid":"0000-0002-3737-9742","position":13,"is_corresponding":false},{"id":218437,"name":"Chien‐Te K. Tseng","orcid":"0000-0001-5726-9834","position":14,"is_corresponding":false},{"id":5952,"name":"Ralph S. Baric","orcid":"0000-0001-6827-8701","position":15,"is_corresponding":false},{"id":267233,"name":"Dimiter S. Dimitrov","orcid":"0000-0002-2258-1024","position":16,"is_corresponding":false},{"id":267221,"name":"Wei Li","orcid":"0000-0002-6960-7404","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:14:08.313144Z","pmid":"32511413","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}