{"doi":"10.1101/2020.05.12.089706","title":"Human follicular CD4 T cell function is defined by specific molecular, positional and TCR dynamic signatures","abstract":"Abstract The orchestrated interaction between follicular helper CD4 T cells (TFH) and germinal center (GC) B cells is crucial for optimal humoral immunity. However, the regulatory mechanisms behind spatial distribution and function of TFH is not well understood. Here, we studied human TFH cells and found that transitioning to a CD57 hi TFH status was associated with distinct positioning in the GC, phenotype, transcriptional signatures, function and downregulation of their T-cell receptor (TCR). Single cell TCR clonotype analysis indicated a unidirectional transition towards the CD57 hi TFH status, which was marked with drastic changes in the nature of immunological synapse formation where peripheral microclusters become dominant. Lack of central supra molecular activation cluster (cSMAC) formation in TFH synapse was associated with enhanced ubiquitination/proteasome activity in these cells. Our data reveal significant aspects of the tissue organization and heterogeneity of follicular adaptive immunity and suggest that CD57 hi TFH cells are endowed with distinctive programming and spatial positioning for optimal GC B cell help. One Sentence Summary human TFH cell heterogeneity","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126675,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.961,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":406188,"name":"Eirini Moysi","orcid":"0000-0002-2070-2902","position":1,"is_corresponding":false},{"id":574561,"name":"Alessandra Noto","orcid":"0000-0003-2813-9488","position":2,"is_corresponding":false},{"id":574562,"name":"Alexander Chassiakos","orcid":"0000-0002-4871-4411","position":3,"is_corresponding":false},{"id":468255,"name":"Khader Ghneim","orcid":"0000-0003-0548-1942","position":4,"is_corresponding":false},{"id":430140,"name":"Sanjana Shah","orcid":"0000-0002-7389-328X","position":5,"is_corresponding":false},{"id":575212,"name":"Vasilis Papaioannou","orcid":null,"position":6,"is_corresponding":false},{"id":407846,"name":"Giulia Fabozzi","orcid":null,"position":7,"is_corresponding":false},{"id":280561,"name":"David R. Ambrozak","orcid":"0000-0003-1038-7328","position":8,"is_corresponding":false},{"id":574563,"name":"Antigoni Poultsidi","orcid":"0000-0001-8804-729X","position":9,"is_corresponding":false},{"id":574564,"name":"Μαρία Ιωάννου","orcid":"0000-0003-2154-9310","position":10,"is_corresponding":false},{"id":575213,"name":"Craig Fenwik","orcid":null,"position":11,"is_corresponding":false},{"id":277146,"name":"Samuel Darko","orcid":"0000-0002-9711-8122","position":12,"is_corresponding":false},{"id":277153,"name":"Daniel C. Douek","orcid":"0000-0001-5575-8634","position":13,"is_corresponding":false},{"id":244983,"name":"Rafick‐Pierre Sékaly","orcid":"0000-0002-7816-4828","position":14,"is_corresponding":false},{"id":41012,"name":"Giuseppe Pantaleo","orcid":"0000-0003-3651-2721","position":15,"is_corresponding":false},{"id":290824,"name":"Richard A. Koup","orcid":"0000-0002-3090-7282","position":16,"is_corresponding":false},{"id":406189,"name":"Constantinos Petrovas","orcid":"0000-0002-5067-5810","position":17,"is_corresponding":false},{"id":574560,"name":"Kartika Padhan","orcid":"0000-0001-9561-4256","position":0,"is_corresponding":true}],"reference_count":65,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}