{"doi":"10.1101/2020.05.11.089342","title":"Phage-DMS: a comprehensive method for fine mapping of antibody epitopes","abstract":"ABSTRACT Understanding the antibody response is critical to developing vaccine and antibody-based therapies and has inspired the recent development of new methods to isolate antibodies. However, methods to define the antibody-antigen interactions that determine specificity or allow escape have not kept pace. We developed Phage-DMS, a method which combines two powerful approaches – immunoprecipitation of phage peptide libraries and deep mutational scanning (DMS) – to enable high-throughput fine mapping of antibody epitopes. As an example, we designed sequences encoding all possible amino acid variants of HIV Envelope to create phage display libraries. Using Phage-DMS, we identified sites of escape predicted using other approaches for four well characterized HIV monoclonal antibodies with known linear epitopes. In some cases, the results of Phage-DMS refined the epitope beyond what was determined in previous studies. This method has the potential to rapidly and comprehensively screen many antibodies in a single experiment to define sites essential for binding to antigen.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126651,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.944,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":486672,"name":"Hannah L. Itell","orcid":"0000-0001-8296-8645","position":1,"is_corresponding":false},{"id":106711,"name":"Katharine H. D. Crawford","orcid":"0000-0002-6223-4019","position":2,"is_corresponding":false},{"id":295731,"name":"Ryan Basom","orcid":"0000-0001-6137-1807","position":3,"is_corresponding":false},{"id":106715,"name":"Jesse D. Bloom","orcid":"0000-0003-1267-3408","position":4,"is_corresponding":false},{"id":344987,"name":"Julie Overbaugh","orcid":"0000-0002-0239-9444","position":5,"is_corresponding":false},{"id":439661,"name":"Meghan Garrett","orcid":"0000-0002-3284-3307","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}