{"doi":"10.1101/2020.04.30.070839","title":"Human and mouse transcriptome profiling identifies cross-species homology in pulmonary and lymph node mononuclear phagocytes","abstract":"Summary The mononuclear phagocyte (MP) system consists of macrophages, monocytes, and dendritic cells (DCs). MP subtypes play distinct functional roles in steady state and inflammatory conditions. Though murine MPs are well characterized, their pulmonary and lymph node (LN) human homologs remain poorly understood. To address this gap, we created a gene expression compendium across 15 distinct human and 9 distinct murine MPs from lung, LN, blood, and spleen. Human blood MPs and murine spleen MPs served as validation datasets, as the human-mouse MP homologs are relatively well-defined in these tissues. In-depth RNA sequencing identified corresponding human-mouse MP subtypes and determined marker genes shared and divergent across between species counterparts. Unexpectedly, at the gene expression level, only 13-23% of the top 1000 marker genes (i.e., genes not shared across species-specific MP subtypes) overlapped in corresponding human-mouse MP counterparts, indicating a need for caution when translating mouse studies to human gene targets and functions. Lastly, CD88 was useful in both species to distinguish macrophage and tissue monocytes from DCs. Our cross-species gene expression compendium serves as a resource for future translational studies to investigate beforehand whether pursuing specific MP subtypes, or genes will prove fruitful.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121188,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.6577,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":297347,"name":"Sophie L. Gibbings","orcid":"0000-0001-9623-0705","position":1,"is_corresponding":false},{"id":303830,"name":"Anita Tewari","orcid":"0000-0001-6331-1701","position":2,"is_corresponding":false},{"id":303831,"name":"Shaikh M. Atif","orcid":"0000-0002-7928-5365","position":3,"is_corresponding":false},{"id":303832,"name":"Brian Vestal","orcid":"0000-0002-3772-1691","position":4,"is_corresponding":false},{"id":297881,"name":"Thomas Danhorn","orcid":"0000-0002-3861-8602","position":5,"is_corresponding":false},{"id":44871,"name":"William J. Janssen","orcid":"0000-0002-6397-3454","position":6,"is_corresponding":false},{"id":226967,"name":"Tor D. Wager","orcid":"0000-0002-1936-5574","position":7,"is_corresponding":false},{"id":61572,"name":"Claudia Jakubzick","orcid":"0000-0002-3731-0198","position":8,"is_corresponding":false},{"id":303829,"name":"Sonia M. Leach","orcid":"0000-0003-2910-069X","position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":null,"created_at":"2026-07-18T23:14:42.734024Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}