{"doi":"10.1101/2020.04.29.069542","title":"Longitudinal Quantification of Metabolites and Macromolecules Reveals Age- and Sex-Related Changes in the Healthy Fischer 344 Rat Brain","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Normal aging is associated with numerous biological changes including altered brain metabolism and tissue chemistry.\n                  <jats:italic>In vivo</jats:italic>\n                  characterization of the neurochemical profile during aging is possible using magnetic resonance spectroscopy, a powerful non-invasive technique capable of quantifying brain metabolites involved in physiological processes that become impaired with age. A prominent macromolecular signal underlies those of brain metabolites and is particularly visible at high fields; parameterization of this signal into components improves quantification and expands the number of biomarkers comprising the neurochemical profile. The present study reports, for the first time, the simultaneous absolute quantification of brain metabolites and individual macromolecules in aging male and female Fischer 344 rats, measured longitudinally using proton magnetic resonance spectroscopy at 7T. We identified age- and sex-related changes in neurochemistry, with prominent differences in metabolites implicated in anaerobic energy metabolism, antioxidant capacity, and neuroprotection, as well as numerous macromolecule changes. These findings contribute to our understanding of the neurobiological processes associated with healthy aging, critical for the proper identification and management of pathological aging trajectories.\n                </jats:p>\n                <jats:sec>\n                  <jats:title>HIGHLIGHTS</jats:title>\n                  <jats:list list-type=\"simple\">\n                    <jats:list-item>\n                      <jats:p>→ Magnetic resonance spectroscopy reveals altered chemistry in the aging rat brain</jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>→ Age- and sex-dependent differences in metabolites and macromolecules are present</jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>→ Metabolites and macromolecules are markers of processes involved in healthy aging</jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        → This study clarifies normative progression of brain chemistry and metabolism\n                        <jats:italic>in vivo</jats:italic>\n                      </jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>→ Improved understanding will inform future studies in pathological aging</jats:p>\n                    </jats:list-item>\n                  </jats:list>\n                </jats:sec>","journal":null,"year":null,"id":660666,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":896925,"name":"Dan Madularu","orcid":"0000-0003-0040-3187","position":1,"is_corresponding":false},{"id":1724676,"name":"Masoumeh Dehghani","orcid":null,"position":2,"is_corresponding":false},{"id":353192,"name":"Gabriel A. Devenyi","orcid":"0000-0002-7766-1187","position":3,"is_corresponding":false},{"id":262953,"name":"Jamie Near","orcid":"0000-0003-3516-936X","position":4,"is_corresponding":false},{"id":1018233,"name":"Caitlin Fowler","orcid":"0000-0003-0159-2637","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Longitudinal Quantification of Metabolites and Macromolecules Reveals Age- and Sex-Related Changes in the Healthy Fischer 344 Rat Brain","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Normal aging is associated with numerous biological changes including altered brain metabolism and tissue chemistry.\n                  <jats:italic>In vivo</jats:italic>\n                  characterization of the neurochemical profile during aging is possible using magnetic resonance spectroscopy, a powerful non-invasive technique capable of quantifying brain metabolites involved in physiological processes that become impaired with age. A prominent macromolecular signal underlies those of brain metabolites and is particularly visible at high fields; parameterization of this signal into components improves quantification and expands the number of biomarkers comprising the neurochemical profile. The present study reports, for the first time, the simultaneous absolute quantification of brain metabolites and individual macromolecules in aging male and female Fischer 344 rats, measured longitudinally using proton magnetic resonance spectroscopy at 7T. We identified age- and sex-related changes in neurochemistry, with prominent differences in metabolites implicated in anaerobic energy metabolism, antioxidant capacity, and neuroprotection, as well as numerous macromolecule changes. These findings contribute to our understanding of the neurobiological processes associated with healthy aging, critical for the proper identification and management of pathological aging trajectories.\n                </jats:p>\n                <jats:sec>\n                  <jats:title>HIGHLIGHTS</jats:title>\n                  <jats:list list-type=\"simple\">\n                    <jats:list-item>\n                      <jats:p>→ Magnetic resonance spectroscopy reveals altered chemistry in the aging rat brain</jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>→ Age- and sex-dependent differences in metabolites and macromolecules are present</jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>→ Metabolites and macromolecules are markers of processes involved in healthy aging</jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        → This study clarifies normative progression of brain chemistry and metabolism\n                        <jats:italic>in vivo</jats:italic>\n                      </jats:p>\n                    </jats:list-item>\n                    <jats:list-item>\n                      <jats:p>→ Improved understanding will inform future studies in pathological aging</jats:p>\n                    </jats:list-item>\n                  </jats:list>\n                </jats:sec>","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W3021092501","authors":[],"funders":[{"funder_name":"Canadian Institutes of Health Research","grant_id":"unidentified","title":"unidentified"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2021,"count":2},{"year":2022,"count":2}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/05/01/2020.04.29.069542.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/05/01/2020.04.29.069542.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2020.04.29.069542","host_type":"publisher"},{"url":"https://doi.org/10.1101/2020.04.29.069542","host_type":"repository"},{"url":"https://doi.org/10.1016/j.neurobiolaging.2020.12.012","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/33610061","host_type":""},{"url":"https://dx.doi.org/10.1016/j.neurobiolaging.2020.12.012","host_type":""},{"url":"https://dx.doi.org/10.1101/2020.04.29.069542","host_type":""},{"url":"http://dx.doi.org/10.1101/2020.04.29.069542","host_type":""}],"fields_of_study":["Advanced MRI Techniques and Applications","Metabolomics and Mass Spectrometry Studies","Mitochondrial Function and Pathology","03 medical and health sciences","0302 clinical medicine"],"mesh_terms":[],"keywords":["Neurochemical","Brain aging","Macromolecule","Neurochemistry","Aging brain","Human brain","Neuroscience","Ageing","In vivo","Chemistry","Metabolism","Biology","Biochemistry","Medicine","Neurology","Internal medicine","Cognition","Male","Sex Characteristics","Magnetic Resonance Spectroscopy","Macromolecular Substances","Brain","Rats, Inbred F344","Rats","Healthy Aging","Animals","Female","Energy Metabolism"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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