{"doi":"10.1101/2020.04.28.066902","title":"The TAT Protein Transduction Domain as an Intra-articular Drug-Delivery Technology","abstract":"Abstract Objective Intra-articular drug delivery holds great promise for the treatment of joint diseases such as osteoarthritis. The objective of this study was to evaluate the TAT peptide transduction domain (TAT-PTD) as a potential intra-articular drug delivery technology for synovial joints. Design Experiments examined the ability of TAT conjugates to associate with primary chondrocytes and alter cellular function both in vitro and in vivo . Further experiments examined the ability of the TAT-PTD to bind to human osteoarthritic cartilage. Results The results show that the TAT-PTD associates with chondrocytes, is capable of delivering siRNA for chondrocyte gene knockdown, and that the recombinant enzyme TAT-Cre is capable of inducing in vivo genetic recombination within the knee joint in a reporter mouse model. Lastly, binding studies show that osteoarthritic cartilage preferentially uptakes the TAT-PTD from solution. Conclusions The results suggest that the TAT-PTD is a promising delivery strategy for intra-articular therapeutics.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126589,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":574449,"name":"Matthew A. Thompson","orcid":"0000-0002-4843-3513","position":1,"is_corresponding":false},{"id":226039,"name":"Akiko Eguchi","orcid":"0000-0002-0555-2707","position":2,"is_corresponding":false},{"id":574450,"name":"Sabrina Dinkel","orcid":"0000-0002-4641-3359","position":3,"is_corresponding":false},{"id":291598,"name":"Martin Lotz","orcid":"0000-0002-6299-8799","position":4,"is_corresponding":false},{"id":227269,"name":"Steven F. Dowdy","orcid":"0000-0003-3083-2257","position":5,"is_corresponding":false},{"id":390815,"name":"Ronald K. June","orcid":"0000-0003-0752-4109","position":6,"is_corresponding":false},{"id":574448,"name":"Sarah Mailhiot","orcid":"0000-0001-7303-0751","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-18T23:15:27.226519Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}