{"doi":"10.1101/2020.04.27.065367","title":"Genetic dissection of the fermentative and respiratory contributions supporting <i>Vibrio cholerae</i> hypoxic growth","abstract":"ABSTRACT Both fermentative and respiratory processes contribute to bacterial metabolic adaptations to low oxygen tension (hypoxia). In the absence of O 2 as a respiratory electron sink, many bacteria utilize alternative electron acceptors such as nitrate (NO 3 − ). During canonical NO 3 − respiration, NO 3 − is reduced in a stepwise manner to N 2 by a dedicated set of reductases. Vibrio cholerae, the etiological agent of cholera, only requires a single periplasmic NO 3 − reductase (NapA) to undergo NO 3 − respiration, suggesting that the pathogen possesses a non-canonical NO 3 − respiratory chain. Here, we used complementary transposon-based screens to identify genetic determinants of general hypoxic growth and NO 3 − respiration in V. cholerae . We found that while the V. cholerae NO 3 − respiratory chain is primarily composed of homologues of established NO 3 − respiratory genes, it also includes components previously unlinked to this process, such as the Na+-NADH dehydrogenase Nqr. The ethanol-generating enzyme AdhE was shown to be the principal fermentative branch required during hypoxic growth in V. cholerae . Relative to single adhE or napA mutant strains, a V. cholerae strain lacking both genes exhibited severely impaired hypoxic growth in vitro and in vivo. Our findings reveal the genetic bases for interactions between disparate energy production pathways that support pathogen fitness in shifting conditions. Such metabolic specializations in V. cholerae and other pathogens are potential targets for antimicrobial interventions. IMPORTANCE Bacteria reprogram their metabolism in environments with low oxygen levels (hypoxia). Typically, this occurs via regulation of two major, but largely independent, metabolic pathways-fermentation and respiration. Here, we found that the diarrheal pathogen Vibrio cholerae has a respiratory chain for NO 3 − that consists largely of components found in other NO 3 − respiratory systems, but also contains several proteins not previously linked to this process. Both AdhE-dependent fermentation and NO 3 − respiration were required for efficient pathogen growth in both laboratory conditions and in an animal infection model. These observations provide genetic evidence for fermentative-respiratory interactions and identify metabolic vulnerabilities that may be targetable for new antimicrobial agents in V. cholerae and related pathogens.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123639,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9533,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":477141,"name":"Brandon Sit","orcid":"0000-0003-2378-3039","position":1,"is_corresponding":false},{"id":332461,"name":"Matthew K. Waldor","orcid":"0000-0003-1843-7000","position":2,"is_corresponding":false},{"id":316673,"name":"Felipe Cava","orcid":"0000-0001-5995-718X","position":3,"is_corresponding":false},{"id":483476,"name":"Emilio Cendejas‐Bueno","orcid":"0000-0002-7796-9026","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-18T23:15:03.403566Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}