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EMTA was found to be suitable for the detection of constitutive activity, inverse agonism, biased signaling and polypharmacology. Profiling of 100 therapeutically relevant human GPCRs resulted in 1,500 pathway-specific concentration-response curves and revealed a great diversity of coupling profiles ranging from exquisite selectivity to broad promiscuity. Overall, this work describes unique resources for studying the complexities underlying GPCR signaling and pharmacology.\n                </jats:p>","journal":null,"year":null,"id":608349,"datarank":0.48283137373023016,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"self_citation_contribution":0.48283137373023016,"citation_network_contribution":0.0,"self_endowment_contribution":0.48283137373023016,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1562386,"name":"Arturo Mancini","orcid":null,"position":1,"is_corresponding":false},{"id":1562387,"name":"Billy Breton","orcid":null,"position":2,"is_corresponding":false},{"id":773863,"name":"Christian Le Gouill","orcid":"0000-0002-6455-1800","position":3,"is_corresponding":false},{"id":670976,"name":"Alexander S. 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Here, we present a set of BRET sensors monitoring the activation of the 12 G protein subtypes based on the translocation of their effectors to the plasma membrane (EMTA). Unlike most of the existing detection systems, EMTA does not require modification of receptors or G proteins (except for G\n                  <jats:sub>s</jats:sub>\n                  ). EMTA was found to be suitable for the detection of constitutive activity, inverse agonism, biased signaling and polypharmacology. Profiling of 100 therapeutically relevant human GPCRs resulted in 1,500 pathway-specific concentration-response curves and revealed a great diversity of coupling profiles ranging from exquisite selectivity to broad promiscuity. Overall, this work describes unique resources for studying the complexities underlying GPCR signaling and pharmacology.\n                </jats:p>","is_dataset_classified":null,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W3205077902","authors":[],"funders":[{"funder_name":"Novo Nordisk Foundation","grant_id":"unidentified","title":"unidentified"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2020,"count":3},{"year":2021,"count":12},{"year":2022,"count":6},{"year":2023,"count":2},{"year":2024,"count":1}],"oa_status":"green","license":"https://www.biorxiv.org/about/FAQ#license","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/04/24/2020.04.20.052027.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/04/24/2020.04.20.052027.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2020.04.20.052027","host_type":"publisher"},{"url":"https://doi.org/10.1101/2020.04.20.052027","host_type":"repository"},{"url":"https://doi.org/10.7554/elife.74101","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/35302493","host_type":""},{"url":"http://dx.doi.org/10.7554/eLife.74101","host_type":""},{"url":"https://doaj.org/article/34e18aa1566b4e768be5932a0e81dff7","host_type":""},{"url":"https://dx.doi.org/10.1101/2020.04.20.052027","host_type":""},{"url":"https://researchprofiles.ku.dk/da/publications/458a7641-d32d-480d-8d94-0fdacae588c1","host_type":""},{"url":"https://curis.ku.dk/ws/files/305174048/elife_74101_v2.pdf","host_type":""},{"url":"https://doi.org/10.7554/eLife.74101","host_type":""},{"url":"https://doi.org/https://doi.org/10.7554/eLife.74101","host_type":""}],"fields_of_study":["Receptor Mechanisms and Signaling","Protein Kinase Regulation and GTPase Signaling","Monoclonal and Polyclonal Antibodies Research","0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":[],"keywords":["G protein-coupled receptor","Effector","Arrestin","Functional selectivity","Receptor","Computational biology","Signal transduction","G protein","Cell biology","Biology","Chemistry","Biochemistry","QH301-705.5","Science","chemical biology","Biosensing Techniques","biosensor","Receptors, G-Protein-Coupled","G protein activation","Biochemistry and Chemical Biology","GTP-Binding Proteins","effector membrane translocation assay","Humans","human","Biology (General)","beta-Arrestins","high-throughput assay","Q","R","HEK293 Cells","beta-Arrestin 1","Medicine","enhanced bystander bioluminescence resonance energy transfer"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T12:06:05.689694Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}