{"doi":"10.1101/2020.04.20.051045","title":"Cadherin-6 mediates thrombosis <i>in vivo</i>","abstract":"Abstract Background Platelet adhesion is the critical process mediating stable thrombus formation. Previous reports of cadherin-6 on human platelets have demonstrated its role in platelet aggregation and thrombus formation. Objectives We aimed to further characterize the importance of cadherin-6 in thrombosis in vivo . Methods Cadherin-6 platelet expression was evaluated by western blotting, flow cytometry and immunoprecipitation. Thrombosis was evaluated using the FeCl 3 and Rose Bengal carotid artery models in C57Bl6 mice treated with anti-cadherin-6 or IgG and wild-type or Cdh6 -/- mice. Platelet function was compared in wild type and Cdh6 -/- mice using tail-clip assays and aggregometry. Results Human platelet expression of cadherin-6 was confirmed at ~3,000 copies per platelet. Cdh6 -/- mice or those treated with anti-Cadherin-6 antibody showed an increased time to occlusion in both thrombosis models. Cadherin-6 was not expressed on mouse platelets, and there were no differences in tail bleeding times or platelet aggregation in wild-type versus Cdh6 -/- mice. Conclusions Cadherin-6 plays an essential role in thrombosis in vivo . However, cadherin-6 is not expressed on murine platelets. These data are in contrast to human platelets, which express a functional cadherin-6/catenin complex. The essential, platelet-independent role for cadherin-6 in hemostasis may allow it to be an effective and safe therapeutic target. Essentials Cadherin-6 function in thrombus formation was investigated in vivo using two murine models of thrombosis. Blocking or deleting cadherin-6 significantly delayed time to occlusion Human platelets express cadherin-6, but murine platelets do not.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126516,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9688,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":573985,"name":"Maria de la Fuente","orcid":"0000-0002-0249-9728","position":1,"is_corresponding":false},{"id":289080,"name":"Elizabeth R. M. Zunica","orcid":"0000-0002-6850-6054","position":2,"is_corresponding":false},{"id":385323,"name":"Wei Li","orcid":"0000-0002-7973-6242","position":3,"is_corresponding":false},{"id":437090,"name":"Michele M. Mumaw","orcid":null,"position":4,"is_corresponding":false},{"id":295123,"name":"Marvin T. Nieman","orcid":"0000-0003-2602-023X","position":5,"is_corresponding":false},{"id":436346,"name":"Emma G. Bouck","orcid":"0000-0002-1481-3059","position":0,"is_corresponding":true}],"reference_count":23,"raw_metadata":null,"created_at":"2026-07-18T23:15:23.509897Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}