{"doi":"10.1101/2020.04.17.045013","title":"Perturbation of <i>in vivo</i> neural activity following α-Synuclein seeding in the olfactory bulb","abstract":"Abstract BACKGROUND Parkinson’s disease (PD) neuropathology is characterized by intraneuronal protein aggregates composed of misfolded α-Synuclein (α-Syn), as well as degeneration of substantia nigra dopamine neurons. Deficits in olfactory perception and aggregation of α-Syn in the olfactory bulb (OB) are observed during early stages of PD, and have been associated with the PD prodrome, before onset of the classic motor deficits. α-Syn fibrils injected into the OB of mice cause progressive propagation of α-Syn pathology throughout the olfactory system and are coupled to olfactory perceptual deficits. OBJECTIVE We hypothesized that accumulation of pathogenic α-Syn in the OB impairs neural activity in the olfactory system. METHODS To address this, we monitored spontaneous and odor-evoked local field potential dynamics in awake wild type mice simultaneously in the OB and piriform cortex (PCX) one, two, and three months following injection of pathogenic preformed α-Syn fibrils in the OB. RESULTS We detected α-Syn pathology in both the OB and PCX. We also observed that α-Syn fibril injections influenced odor-evoked activity in the OB. In particular, α-Syn fibril-injected mice displayed aberrantly high odor-evoked power in the beta spectral range. A similar change in activity was not detected in the PCX, despite high levels of α-Syn pathology. CONCLUSIONS Together, this work provides evidence that synucleinopathy impacts in vivo neural activity in the olfactory system at the network-level.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126532,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9668,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":352092,"name":"María del Mar Cortijo","orcid":null,"position":1,"is_corresponding":false},{"id":520439,"name":"Elizabeth R. Roberts","orcid":"0000-0003-0459-9148","position":2,"is_corresponding":false},{"id":574386,"name":"Tamara L. Suggs","orcid":null,"position":3,"is_corresponding":false},{"id":574387,"name":"Heather B. Stover","orcid":null,"position":4,"is_corresponding":false},{"id":574009,"name":"José I. Pena-Bravo","orcid":"0000-0002-1307-3626","position":5,"is_corresponding":false},{"id":555628,"name":"Jennifer A. Steiner","orcid":"0000-0003-0953-1310","position":6,"is_corresponding":false},{"id":258844,"name":"Kelvin C. Luk","orcid":"0000-0002-6591-6269","position":7,"is_corresponding":false},{"id":255910,"name":"Patrik Brundin","orcid":"0000-0003-2924-5186","position":8,"is_corresponding":false},{"id":306845,"name":"Daniel W. Wesson","orcid":"0000-0002-3192-3866","position":9,"is_corresponding":false},{"id":302828,"name":"Aishwarya Kulkarni","orcid":"0000-0001-5365-214X","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-18T23:15:23.509897Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}