{"doi":"10.1101/2020.04.15.20066746","title":"Temporal detection and phylogenetic assessment of SARS-CoV-2 in municipal wastewater","abstract":"ABSTRACT SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 52-day time course. We show that changes in SARS-CoV-2 RNA concentrations correlate with local COVID-19 epidemiological data (R 2 =0.9), though detection in wastewater trails symptom onset dates by 5-8 days. We determine a near complete (98.5%) SARS-CoV-2 genome sequence from the wastewater and use phylogenic analysis to infer viral ancestry. Collectively, this work demonstrates how wastewater can be used as a proxy to monitor viral prevalence in the community and how genome sequencing can be used for high-resolution genotyping of the predominant strains circulating in a community.","journal":"medRxiv","year":2020,"id":118240,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":188,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":226295,"name":"Anna Nemudraia","orcid":"0000-0002-4190-6306","position":1,"is_corresponding":false},{"id":226296,"name":"Tanner Wiegand","orcid":"0000-0002-0528-268X","position":2,"is_corresponding":false},{"id":226297,"name":"Kevin Surya","orcid":"0000-0003-1536-5929","position":3,"is_corresponding":false},{"id":226298,"name":"Murat Buyukyoruk","orcid":"0000-0003-1534-7202","position":4,"is_corresponding":false},{"id":226299,"name":"Karl K. Vanderwood","orcid":"0009-0002-9994-2374","position":5,"is_corresponding":false},{"id":226300,"name":"Royce A. Wilkinson","orcid":"0000-0001-8831-2081","position":6,"is_corresponding":false},{"id":226301,"name":"Blake Wiedenheft","orcid":"0000-0001-9297-5304","position":7,"is_corresponding":false},{"id":226294,"name":"Artem Nemudryi","orcid":"0000-0003-0121-1363","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-18T23:13:58.531532Z","pmid":"32511611","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}