{"doi":"10.1101/2020.04.15.039933","title":"Proteomics of protein trafficking by <i>in vivo</i> tissue-specific labeling","abstract":"Abstract Secreted interorgan communication factors encode key regulators of homeostasis. However, long-standing questions surround their origins/destinations, mechanisms of interactions, and the number of proteins involved. Progress has been hindered by the lack of methodologies for these factors’ large-scale identification and characterization, as conventional approaches cannot identify low-abundance factors and the origins and destinations of secreted proteins. We established an in vivo platform to investigate secreted protein trafficking between organs proteome-wide, whereby engineered promiscuous biotin ligase BirA*G3 (a relative of TurboID) biotinylates all proteins in a subcellular compartment of one tissue, and biotinylated proteins are affinity-enriched and identified from distal organs using quantitative mass spectrometry. Using this platform, we identified 51 putative muscle-secreted proteins from heads and 269 fat body-secreted proteins from legs/muscles, of which 60-70% have human orthologs. We demonstrate, in particular, that conserved fat body-derived novel interorgan communication factors CG31326, CG2145, and CG4332 promote muscle activity. Our results indicate that the communication network of secreted proteins is vast, and we identified systemic functions for a number of these factors. This approach is widely applicable to studies in interorgan, local and intracellular protein trafficking networks, non-conventional secretion, and to mammalian systems, under healthy or diseased states. One Sentence Summary We developed an in vivo platform to investigate protein trafficking between organs proteome-wide, provide a resource for interorgan communication factors, and determined conserved adipokines that affect muscles.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":122701,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9455,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":564454,"name":"Dan Wang","orcid":"0000-0003-2881-2273","position":1,"is_corresponding":false},{"id":259031,"name":"Yanhui Hu","orcid":"0000-0003-1494-1402","position":2,"is_corresponding":false},{"id":6645,"name":"Namrata D. Udeshi","orcid":"0000-0001-5312-1402","position":3,"is_corresponding":false},{"id":564455,"name":"Luye Mu","orcid":"0000-0002-6810-7598","position":4,"is_corresponding":false},{"id":242236,"name":"Tanya Svinkina","orcid":"0009-0005-8300-3403","position":5,"is_corresponding":false},{"id":376811,"name":"Rebecca Zeng","orcid":"0000-0002-1186-4758","position":6,"is_corresponding":false},{"id":552756,"name":"Tess C. Branon","orcid":"0000-0002-6520-6419","position":7,"is_corresponding":false},{"id":564456,"name":"Areya Tabatabai","orcid":"0000-0002-4480-9245","position":8,"is_corresponding":false},{"id":264325,"name":"Justin A. Bosch","orcid":"0000-0001-8499-1566","position":9,"is_corresponding":false},{"id":3257,"name":"John M. Asara","orcid":"0000-0001-7450-2589","position":10,"is_corresponding":false},{"id":242245,"name":"Alice Y. Ting","orcid":"0000-0002-8277-5226","position":11,"is_corresponding":false},{"id":2226,"name":"Steven A. Carr","orcid":"0000-0002-7203-4299","position":12,"is_corresponding":false},{"id":14957,"name":"Norbert Perrimon","orcid":"0000-0001-7542-472X","position":13,"is_corresponding":false},{"id":564453,"name":"Ilia A. Droujinine","orcid":"0000-0003-1311-4392","position":0,"is_corresponding":true}],"reference_count":25,"raw_metadata":null,"created_at":"2026-07-18T23:14:55.385653Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}