{"doi":"10.1101/2020.04.09.034280","title":"A Systematic Analysis of Mosquito-Microbiome Biosynthetic Gene Clusters Reveals Antimalarial Siderophores that Reduce Mosquito Reproduction Capacity","abstract":"ABSTRACT Advances in infectious disease control strategies through genetic manipulation of insect microbiomes have heightened interest in microbially produced small molecules within mosquitoes. Herein, 33 mosquito-associated bacterial genomes were mined and over 700 putative biosynthetic gene clusters (BGCs) were identified, 135 of which belong to known classes of BGCs. After an in-depth analysis of the 135 BGCs, iron-binding siderophores were chosen for further investigation due to their high abundance and well-characterized bioactivities. Through various metabolomic strategies, eight siderophore scaffolds were identified in six strains of mosquito-associated bacteria. Among these, serratiochelin A and pyochelin were found to reduce female Anopheles gambiae overall fecundity likely by lowering their blood feeding rate. Serratiochelin A and pyochelin were further found to inhibit the Plasmodium parasite asexual blood and liver stages in vitro . Our work supplies a bioinformatic resource for future mosquito microbiome studies and highlights an understudied source of bioactive small molecules.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":124768,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.945,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":449667,"name":"Ashmita Pandey","orcid":"0000-0001-5461-7768","position":1,"is_corresponding":false},{"id":355428,"name":"Kayla Sylvester","orcid":"0000-0003-4570-7432","position":2,"is_corresponding":false},{"id":330974,"name":"Kuan‐Yi Lu","orcid":"0000-0002-3663-377X","position":3,"is_corresponding":false},{"id":569979,"name":"Maria Toro-Moreno","orcid":"0000-0002-0497-2259","position":4,"is_corresponding":false},{"id":570525,"name":"Sina Rütschlin","orcid":null,"position":5,"is_corresponding":false},{"id":369680,"name":"James M. Bradford","orcid":"0000-0003-0730-152X","position":6,"is_corresponding":false},{"id":326563,"name":"Cody Champion","orcid":"0000-0003-0619-905X","position":7,"is_corresponding":false},{"id":569980,"name":"Thomas Böttcher","orcid":"0000-0003-0235-4825","position":8,"is_corresponding":false},{"id":326553,"name":"Jiannong Xu","orcid":"0000-0001-7332-4370","position":9,"is_corresponding":false},{"id":330975,"name":"Emily R. Derbyshire","orcid":"0000-0001-6664-8844","position":10,"is_corresponding":false},{"id":569978,"name":"Jack G. Ganley","orcid":"0000-0002-1396-439X","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-18T23:15:11.632153Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}