{"doi":"10.1101/2020.04.08.032433","title":"Improved genetically encoded near-infrared fluorescent calcium ion indicators for <i>in vivo</i> imaging","abstract":"Abstract Near-infrared (NIR) genetically-encoded calcium ion (Ca 2+ ) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross-talk with visible-light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report two improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and C. elegans and Xenopus laevis in vivo . Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca 2+ dynamics.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121162,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":282658,"name":"Danielle M. Cosio","orcid":"0000-0003-2997-4813","position":1,"is_corresponding":false},{"id":11616,"name":"Kiryl D. Piatkevich","orcid":"0000-0002-7777-9468","position":2,"is_corresponding":false},{"id":282659,"name":"Sarah Aufmkolk","orcid":"0000-0002-0745-6022","position":3,"is_corresponding":false},{"id":283738,"name":"Wan-Chi Su","orcid":null,"position":4,"is_corresponding":false},{"id":264902,"name":"Orhan T. Celiker","orcid":null,"position":5,"is_corresponding":false},{"id":282660,"name":"Anne Schohl","orcid":"0000-0002-6828-5490","position":6,"is_corresponding":false},{"id":11595,"name":"Mitchell H. Murdock","orcid":"0000-0001-8625-624X","position":7,"is_corresponding":false},{"id":282661,"name":"Abhi Aggarwal","orcid":"0000-0002-6336-566X","position":8,"is_corresponding":false},{"id":282662,"name":"Yu‐Fen Chang","orcid":"0000-0002-9147-9162","position":9,"is_corresponding":false},{"id":282663,"name":"Paul W. Wiseman","orcid":"0000-0002-5732-2858","position":10,"is_corresponding":false},{"id":282664,"name":"Edward S. Ruthazer","orcid":"0000-0003-0452-3151","position":11,"is_corresponding":false},{"id":11617,"name":"Edward S. Boyden","orcid":"0000-0002-0419-3351","position":12,"is_corresponding":false},{"id":197736,"name":"Robert E Campbell","orcid":"0000-0003-0604-092X","position":13,"is_corresponding":false},{"id":11612,"name":"Yong Qian","orcid":"0000-0001-7849-9022","position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-18T23:14:42.734024Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}