{"doi":"10.1101/2020.04.01.019877","title":"Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo","abstract":"SARS-CoV-2 has rapidly become a pandemic worldwide; therefore, an effective vaccine is urgently needed. Recently, messenger RNAs (mRNAs) have emerged as a promising platform for vaccination. Here, we systematically investigated the untranslated regions (UTRs) of mRNAs in order to enhance protein production. Through a comprehensive analysis of endogenous gene expression and de novo design of UTRs, we identified the optimal combination of 5' and 3' UTR, termed as NASAR, which was five to ten-fold more efficient than the tested endogenous UTRs. More importantly, NASAR mRNAs delivered by lipid-derived nanoparticles showed dramatic expression of potential SARS-CoV-2 antigens both in vitro and in vivo. These NASAR mRNAs merit further development as alternative SARS-CoV-2 vaccines.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":119002,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":257716,"name":"Xucheng Hou","orcid":"0000-0001-7833-8704","position":1,"is_corresponding":false},{"id":257717,"name":"Jinyue Yan","orcid":"0000-0003-0300-0762","position":2,"is_corresponding":false},{"id":257718,"name":"Chengxiang Zhang","orcid":"0009-0008-4858-7513","position":3,"is_corresponding":false},{"id":257719,"name":"Wenqing Li","orcid":"0000-0002-8336-5552","position":4,"is_corresponding":false},{"id":257720,"name":"Weiyu Zhao","orcid":"0000-0003-3345-4925","position":5,"is_corresponding":false},{"id":260884,"name":"Shi Du","orcid":null,"position":6,"is_corresponding":false},{"id":255309,"name":"Yizhou Dong","orcid":"0000-0001-5786-0659","position":7,"is_corresponding":false},{"id":257715,"name":"Chunxi Zeng","orcid":"0000-0002-1218-1908","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-18T23:14:03.409507Z","pmid":"32511313","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}