{"doi":"10.1101/2020.03.29.002857","title":"Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time","abstract":"ABSTRACT Phagocytosis by glial cells is essential to regulate brain function during development and disease. Given recent interest in using amyloid β (Aβ)-targeted antibodies as a therapy for patients with Alzheimer’s disease, removal of Aβ by phagocytosis is likely protective early in Alzheimer’s disease, but remains poorly understood. Impaired phagocytic function of glial cells surrounding Aβ plaques during later stages in Alzheimer’s disease likely contributes to worsened disease outcomes, but the underlying mechanisms of how this occurs remain unknown. We have developed a human Aβ 1-42 analogue (Aβ pH ) that exhibits green fluorescence upon internalization into the acidic phagosomes of cells but is non-fluorescent at physiological pH. This allowed us to image, for the first time, glial uptake of Aβ pH in real time in live animals. Microglia phagocytose more Aβ pH than astrocytes in culture, in brain slices and in vivo . Aβ pH can be used to investigate the phagocytic mechanisms removing Aβ from the extracellular space, and thus could become a useful tool to study Aβ clearance at different stages of Alzheimer’s disease.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123549,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.951,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":560132,"name":"Krupal P. Jethava","orcid":"0000-0002-4440-3333","position":1,"is_corresponding":false},{"id":566444,"name":"Nils Korte","orcid":"0000-0002-7893-8432","position":2,"is_corresponding":false},{"id":566445,"name":"Pablo Izquierdo","orcid":"0000-0003-4372-348X","position":3,"is_corresponding":false},{"id":566446,"name":"Emilia Favuzzi","orcid":"0000-0003-4256-1490","position":4,"is_corresponding":false},{"id":566447,"name":"Indigo V.L. Rose","orcid":"0000-0003-0491-1269","position":5,"is_corresponding":false},{"id":68252,"name":"Kevin A. Guttenplan","orcid":"0000-0003-4242-4349","position":6,"is_corresponding":false},{"id":320966,"name":"Sayan Deb Dutta","orcid":"0000-0001-9397-3140","position":7,"is_corresponding":false},{"id":320969,"name":"Jean‐Christophe Rochet","orcid":"0000-0002-6484-1541","position":8,"is_corresponding":false},{"id":246021,"name":"Gord Fishell","orcid":"0000-0002-9640-9278","position":9,"is_corresponding":false},{"id":29917,"name":"Shane A. Liddelow","orcid":"0000-0002-0840-1437","position":10,"is_corresponding":false},{"id":566448,"name":"David Attwell","orcid":"0000-0003-3618-0843","position":11,"is_corresponding":false},{"id":345364,"name":"Gaurav Chopra","orcid":"0000-0003-0942-7898","position":12,"is_corresponding":false},{"id":566443,"name":"Priya Prakash","orcid":"0000-0003-2193-9669","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-18T23:14:59.547352Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}