{"doi":"10.1101/2020.03.23.004325","title":"Glycoengineering of NK cells with glycan ligands of CD22 and selectins for B-cell lymphoma therapy","abstract":"ABSTRACT CD22, a member of Siglec family of sialic acid binding proteins, has restricted expression on B cells. Antibody-based agents targeting CD22 or CD20 (Rituxan™) on B lymphoma and leukemia cells exhibit clinical efficacy for treating these malignancies, but also attack normal B cells leading to immune deficiency. Here, we report a chemoenzymatic glycocalyx editing strategy to introduce high-affinity and specific CD22 ligands onto NK-92MI and cytokine-induced killer (CIK) cells to achieve tumor-specific CD22 targeting. These CD22-ligand modified cells exhibited significantly enhanced tumor cell binding and killing in vitro without harming healthy B cells. For effective lymphoma cell killing in vivo we further functionalized CD22 ligand-modified NK-92MI cells with the E-selectin ligand sialyl Lewis X to promote trafficking to bone marrow. The cells containing the ligands of both CD22 and selectins resulted in the efficient suppression of B lymphoma in a xenograft model. Our results suggest that NK cells modified with glycan ligands to CD22 and selectins promote both targeted killing of B lymphoma cells and improved trafficking to sites where the cancer cells reside, respectively.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121520,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":297065,"name":"Chenhua Yu","orcid":null,"position":1,"is_corresponding":false},{"id":270756,"name":"Peng Wang","orcid":"0009-0009-0391-7379","position":2,"is_corresponding":false},{"id":262638,"name":"Yujie Shi","orcid":"0000-0002-7610-0433","position":3,"is_corresponding":false},{"id":296104,"name":"Bo Cheng","orcid":"0000-0003-3654-9962","position":4,"is_corresponding":false},{"id":262636,"name":"Mingkuan Chen","orcid":"0000-0002-0878-0545","position":5,"is_corresponding":false},{"id":296105,"name":"Digantkumar Chapla","orcid":"0000-0003-0649-1368","position":6,"is_corresponding":false},{"id":561459,"name":"Natalie Reigh","orcid":null,"position":7,"is_corresponding":false},{"id":296108,"name":"Yoshiki Narimatsu","orcid":"0000-0003-1428-5695","position":8,"is_corresponding":false},{"id":533088,"name":"Xing Chen","orcid":"0000-0002-3058-7370","position":9,"is_corresponding":false},{"id":296110,"name":"Henrik Clausen","orcid":"0000-0002-0915-5055","position":10,"is_corresponding":false},{"id":297066,"name":"Kelly W. Moremen","orcid":null,"position":11,"is_corresponding":false},{"id":296111,"name":"Matthew S. Macauley","orcid":"0000-0003-4579-1048","position":12,"is_corresponding":false},{"id":230746,"name":"James C. Paulson","orcid":"0000-0003-4589-5322","position":13,"is_corresponding":false},{"id":262641,"name":"Peng Wu","orcid":"0000-0002-5204-0229","position":14,"is_corresponding":false},{"id":296102,"name":"Senlian Hong","orcid":"0000-0002-4082-9767","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-18T23:14:42.734024Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}