{"doi":"10.1101/2020.03.13.991414","title":"Efficient Generation of Isogenic Primary Human Myeloid Cells using CRISPR-Cas9 Ribonucleoproteins","abstract":"Summary Genome engineering of primary human cells with CRISPR-Cas9 has revolutionized experimental and therapeutic approaches to cell biology, but human myeloid-lineage cells have remained largely genetically intractable. We present a method for delivery of CRISPR-Cas9 ribonucleoprotein (RNP) complexes by nucleofection directly into CD14+ human monocytes purified from peripheral blood, leading to high rates of precise gene knockout. These cells can be efficiently differentiated into monocyte-derived macrophages or dendritic cells. This process yields genetically-edited cells that retain critical markers of both myeloid differentiation and phagocytic function. Genetic ablation of the restriction factor SAMHD1 increased HIV-1 infection more than fifty-fold, demonstrating the power of this system for genotype-phenotype interrogation. This fast, flexible and scalable platform can be used for genetic studies of human myeloid cells in immune signaling, inflammation, cancer immunology, host-pathogen interactions, and beyond, and could facilitate development of novel myeloid cellular therapies.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":121147,"datarank":0.4981183817832415,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.18620215053126607,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.18620215053126607,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":7,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9418,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":103692,"name":"Devin A. Cavero","orcid":"0000-0002-3830-6625","position":1,"is_corresponding":false},{"id":103639,"name":"Michael McGregor","orcid":"0000-0001-5281-5736","position":2,"is_corresponding":false},{"id":103626,"name":"David E. Gordon","orcid":"0000-0001-7954-0306","position":3,"is_corresponding":false},{"id":560242,"name":"Weihao Zheng","orcid":"0000-0002-8038-1207","position":4,"is_corresponding":false},{"id":342866,"name":"Jonathan M. Budzik","orcid":"0000-0001-8025-7911","position":5,"is_corresponding":false},{"id":103693,"name":"Theodore L. Roth","orcid":"0000-0002-3970-9573","position":6,"is_corresponding":false},{"id":103633,"name":"Kelsey M. Haas","orcid":"0000-0002-1463-6726","position":7,"is_corresponding":false},{"id":103694,"name":"Ujjwal Rathore","orcid":"0000-0002-0329-7431","position":8,"is_corresponding":false},{"id":107226,"name":"Anke Meyer‐Franke","orcid":null,"position":9,"is_corresponding":false},{"id":537094,"name":"Mohamed S. Bouzidi","orcid":null,"position":10,"is_corresponding":false},{"id":36741,"name":"Judd F. Hultquist","orcid":"0000-0001-6424-4280","position":11,"is_corresponding":false},{"id":225085,"name":"Jason A. Wojcechowskyj","orcid":"0000-0001-6394-4840","position":12,"is_corresponding":false},{"id":560243,"name":"Krystal A. Fontaine","orcid":"0000-0002-0793-9163","position":13,"is_corresponding":false},{"id":24132,"name":"Satish K. Pillai","orcid":"0000-0001-8770-7014","position":14,"is_corresponding":false},{"id":251792,"name":"Jeffery S. Cox","orcid":"0000-0002-5061-6618","position":15,"is_corresponding":false},{"id":8685,"name":"J. Ernst","orcid":"0000-0001-9951-6207","position":16,"is_corresponding":false},{"id":1363,"name":"Nevan J. Krogan","orcid":"0000-0003-4902-337X","position":17,"is_corresponding":false},{"id":30775,"name":"Alexander Marson","orcid":"0000-0002-2734-5776","position":18,"is_corresponding":false},{"id":1476,"name":"Joseph Hiatt","orcid":"0000-0002-8015-9614","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:14:42.734024Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}