{"doi":"10.1101/2020.03.10.986703","title":"Identification of <i>Candida glabrata</i> Transcriptional Regulators that Govern Stress Resistance and Virulence","abstract":"Abstract The mechanisms by which Candida glabrata resists host defense peptides and caspofungin are incompletely understood. To identify transcriptional regulators that enable C. glabrata to withstand these classes of stressors, a library of 215 C. glabrata transcriptional regulatory deletion mutants was screened for susceptibility to both protamine and caspofungin. We identified 8 mutants that had increased susceptibility to both host defense peptides and caspofungin. Of these mutants, 6 were deleted for genes that were predicted to specify proteins involved in histone modification. These genes were ADA2, GCN5, SPT8, HOS2, RPD3 , and SPP1 . The ada2 Δ and gcn5 Δ mutants also had increased susceptibility to other stressors such as H 2 O 2 and SDS. In the Galleria mellonella model of disseminated infection, the ada2 Δ and gcn5 Δ mutants had attenuated virulence, whereas in neutropenic mice, the virulence of the ada2 Δ and rpd3 Δ mutants was decreased. Thus, histone modification plays a central role in enabling C. glabrata to withstand host defense peptides and caspofungin, and Ada2 is essential for the maximal virulence of this organism during disseminated infection.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":129123,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9435,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":580347,"name":"Yaoping Liu","orcid":"0000-0002-3881-1904","position":1,"is_corresponding":false},{"id":314446,"name":"Norma V. Solis","orcid":"0000-0001-5163-2110","position":2,"is_corresponding":false},{"id":580348,"name":"Luis Diaz","orcid":"0000-0002-7744-502X","position":3,"is_corresponding":false},{"id":308240,"name":"John E. Edwards","orcid":"0000-0001-7608-6021","position":4,"is_corresponding":false},{"id":294224,"name":"Scott G. Filler","orcid":"0000-0001-7278-3700","position":5,"is_corresponding":false},{"id":345685,"name":"Michael R. Yeaman","orcid":"0000-0002-9468-5252","position":6,"is_corresponding":false},{"id":580819,"name":"Elan E. Filler","orcid":null,"position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-18T23:15:45.920615Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}