{"doi":"10.1101/2020.03.10.984922","title":"Molecular basis of ubiquitination catalyzed by the bacterial transglutaminase MavC","abstract":"Summary The Legionella pneumophila effector MavC is a transglutaminase that carries out atypical ubiquitination of the ubiquitin (Ub) E2 conjugation enzyme UBE2N by catalyzing the formation of an isopeptide bond between Gln40 of Ub and Lys92 (or to a less extent, Lys94) of UBE2N, which results in inhibition of UBE2N signaling in the NF-κB pathway. In the absence of UBE2N, MavC deamidates Ub at Gln40 or catalyzes self-ubiquitination. However, the mechanisms underlying these enzymatic activities of MavC are not fully understood at molecular level. In this study, we obtained the structure of the MavC-UBE2N-Ub ternary complex that represents a snapshot of covalent cross-linking of UBE2N and Ub catalyzed by MavC. The structure reveals the unique way by which the cross-linked catalytic product UBE2N-Ub binds mainly to the Insertion and the Tail domains of MavC prior to its release. Based on our structural, biochemical and mutational analyses, we proposed the catalytic mechanism for both the deamidase and the transglutaminase activities of MavC. Finally, by comparing the structures of MavC and MvcA, the homologous protein that reverses MavC-induced UBE2N ubiquitination, we identified several key regions of the two proteins responsible for their opposite enzymatic activity. Our results provide insights into the mechanisms for substrate recognition and ubiquitination mediated by MavC as well as explanations for the opposite activity of MavC and MvcA.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123536,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9551,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":395229,"name":"Jiaqi Fu","orcid":"0000-0003-0081-6133","position":1,"is_corresponding":false},{"id":395230,"name":"Ting Yu","orcid":"0000-0002-3644-7467","position":2,"is_corresponding":false},{"id":395231,"name":"Zhao‐Xi Wang","orcid":"0000-0002-2689-7034","position":3,"is_corresponding":false},{"id":395232,"name":"Ninghai Gan","orcid":"0000-0001-7238-4056","position":4,"is_corresponding":false},{"id":396379,"name":"Yini Huang","orcid":null,"position":5,"is_corresponding":false},{"id":395233,"name":"Vanja Perčulija","orcid":"0000-0003-1075-188X","position":6,"is_corresponding":false},{"id":395234,"name":"Li Yu","orcid":"0000-0001-8820-6705","position":7,"is_corresponding":false},{"id":395235,"name":"Zhao‐Qing Luo","orcid":"0000-0001-8890-6621","position":8,"is_corresponding":false},{"id":395236,"name":"Songying Ouyang","orcid":"0000-0002-1120-1524","position":9,"is_corresponding":false},{"id":395228,"name":"Hongxin Guan","orcid":"0009-0005-5469-8840","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:14:59.547352Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}