{"doi":"10.1101/2020.03.06.981472","title":"Converting lateral scanning into axial focusing to speed up 3D microscopy","abstract":"Abstract In optical microscopy, the slow axial scanning rate of the objective or the sample has traditionally limited the speed of 3D volumetric imaging. Recently, by conjugating either a movable-mirror to the image plane or an electrotuneable lens (ETL) to the back-focal plane respectively, rapid axial scanning has been achieved. However, mechanical actuation of a mirror limits axial scanning rate (usually only 10-100 Hz for piezoelectric or voice coil based actuators), while ETLs introduce spherical and higher order aberrations, thereby preventing high-resolution imaging. Here, we introduce a novel optical design that can transform a lateral-scan motion into a spherical-aberration-free, high-resolution, rapid axial scan. Using a galvanometric mirror, we scan a laser beam laterally in a remote-focusing arm, which is then back-reflected from different heights of a mirror in image space. We characterize the optical performance of this remote focusing technique and use it to accelerate axially swept light-sheet microscopy (ASLM) by one order of magnitude, allowing the quantification of rapid vesicular dynamics in 3D.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123503,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9495,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":245868,"name":"Bo-Jui Chang","orcid":"0000-0002-5513-7106","position":1,"is_corresponding":false},{"id":335040,"name":"Stephan Daetwyler","orcid":"0000-0002-7444-4734","position":2,"is_corresponding":false},{"id":245867,"name":"Etai Sapoznik","orcid":"0000-0001-8472-0299","position":3,"is_corresponding":false},{"id":353381,"name":"Bingying Chen","orcid":"0000-0003-3518-6290","position":4,"is_corresponding":false},{"id":245883,"name":"Kevin M. Dean","orcid":"0000-0003-0839-2320","position":5,"is_corresponding":false},{"id":225169,"name":"Reto Fiolka","orcid":"0000-0002-4636-5000","position":6,"is_corresponding":false},{"id":353380,"name":"Tonmoy Chakraborty","orcid":"0000-0001-7956-1932","position":0,"is_corresponding":true}],"reference_count":19,"raw_metadata":null,"created_at":"2026-07-18T23:14:59.547352Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}