{"doi":"10.1101/2020.03.05.977884","title":"Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  We analyzed all four Pex23 family proteins of the yeast\n                  <jats:italic>Hansenula polymorpha</jats:italic>\n                  , which localize to the ER. Of these Pex24 and Pex32, but not Pex23 and Pex29, accumulate at peroxisome-ER contacts, where they are important for normal peroxisome biogenesis and proliferation and contribute to organelle positioning and segregation.\n                </jats:p>\n                <jats:p>\n                  Upon deletion of\n                  <jats:italic>PEX24</jats:italic>\n                  and\n                  <jats:italic>PEX32</jats:italic>\n                  - and to a lesser extent of\n                  <jats:italic>PEX23</jats:italic>\n                  and\n                  <jats:italic>PEX29</jats:italic>\n                  - peroxisome-ER contacts are disrupted, concomitant with peroxisomal defects. These defects are suppressed upon introduction of an artificial peroxisome-ER tether.\n                </jats:p>\n                <jats:p>Accumulation of Pex32 at peroxisomes-ER contacts is lost in the absence of the peroxisomal membrane protein Pex11. At the same time peroxisome-ER contacts are disrupted, indicating that Pex11 contributes to Pex32-dependent peroxisome-ER contact formation.</jats:p>\n                <jats:p>\n                  Summarizing, our data indicate that\n                  <jats:italic>H. polymorpha</jats:italic>\n                  Pex24 and Pex32 are tethers at peroxisome-ER contacts that are important for normal peroxisome biogenesis and dynamics.\n                </jats:p>\n                <jats:sec>\n                  <jats:title>Summary</jats:title>\n                  <jats:p>\n                    Two\n                    <jats:italic>Hansenula polymorpha</jats:italic>\n                    ER proteins, Pex24 and Pex32, are tethers at peroxisome-ER contacts and function together with the peroxisomal protein Pex11. Their absence disturbs these contacts leading to multiple peroxisomal defects, which can be restored by an artificial tether.\n                  </jats:p>\n                </jats:sec>","journal":null,"year":null,"id":599211,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1535724,"name":"Rinse de Boer","orcid":null,"position":1,"is_corresponding":false},{"id":1535725,"name":"Arjen M. Krikken","orcid":null,"position":2,"is_corresponding":false},{"id":1535726,"name":"Arman Akşit","orcid":null,"position":3,"is_corresponding":false},{"id":689889,"name":"Nicola Bordin","orcid":"0000-0002-6568-9035","position":4,"is_corresponding":false},{"id":1535727,"name":"Damien P. Devos","orcid":null,"position":5,"is_corresponding":false},{"id":1535728,"name":"Ida J. van der Klei","orcid":"0000-0001-7165-9679","position":6,"is_corresponding":false},{"id":1145983,"name":"Fei Wu","orcid":"0000-0001-5388-0003","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation","abstract":"We analyzed all four Pex23 family proteins of the yeast  Hansenula polymorpha , which localize to the ER. Of these Pex24 and Pex32, but not Pex23 and Pex29, accumulate at peroxisome-ER contacts, where they are important for normal peroxisome biogenesis and proliferation and contribute to organelle positioning and segregation.  Upon deletion of  PEX24 and  PEX32 - and to a lesser extent of  PEX23 and  PEX29 - peroxisome-ER contacts are disrupted, concomitant with peroxisomal defects. These defects are suppressed upon introduction of an artificial peroxisome-ER tether.  Accumulation of Pex32 at peroxisomes-ER contacts is lost in the absence of the peroxisomal membrane protein Pex11. At the same time peroxisome-ER contacts are disrupted, indicating that Pex11 contributes to Pex32-dependent peroxisome-ER contact formation. Summarizing, our data indicate that  H. polymorpha Pex24 and Pex32 are tethers at peroxisome-ER contacts that are important for normal peroxisome biogenesis and dynamics.  <h4>Summary</h4>  Two  Hansenula polymorpha ER proteins, Pex24 and Pex32, are tethers at peroxisome-ER contacts and function together with the peroxisomal protein Pex11. Their absence disturbs these contacts leading to multiple peroxisomal defects, which can be restored by an artificial tether.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"European Commission","grant_id":"316723","title":"Peroxisomes: key to cell performance and health"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"https://www.biorxiv.org/about/FAQ#license","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2020/03/06/2020.03.05.977884.full.pdf","host_type":"repository"},{"url":"https://doi.org/10.1101/2020.03.05.977884","host_type":""},{"url":"https://jcs.biologists.org/content/joces/133/16/jcs246983.full.pdf","host_type":""},{"url":"https://dx.doi.org/10.1101/2020.03.05.977884","host_type":""},{"url":"http://dx.doi.org/10.1101/2020.03.05.977884","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T19:49:53.839583Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}