{"doi":"10.1101/2020.03.04.977280","title":"Satellite cell-derived extracellular vesicles reverse peroxide-induced mitochondrial dysfunction in myotubes","abstract":"ABSTRACT Satellite cells (SCs) are muscle-specific stem cells that have a central role in muscle remodeling. Despite their therapeutic potential, SC-based therapies have been met with numerous logistical challenges, limiting their ability to effectively treat systemic muscle diseases, such as Duchenne muscular dystrophy (DMD). Delivery of SC-derived extracellular vesicles (SC-EVs) may unlock the potential offered by SCs and overcome their numerous limitations. Purpose The purpose of this investigation was to determine the extent to which SC-EVs could restore mitochondrial function in cultured myotubes following oxidative injury. Methods SC-EVs were isolated from cultured SCs from C57 mice and quantified using nanoparticle tracking analysis (NTA). C2C12 myotubes were cultured and divided into four treatment groups: untreated control, treated for 24 h with SC-EV, 24 h exposure to 50 μM H 2 O 2 followed by a 24 h recovery period with no treatment, or 24 h exposure to 50 μM H 2 O 2 followed by a 24 h treatment with SC-EV. Inter-group differences in mitochondrial function were assessed via one-way ANOVA with Tukey post hoc analysis (p<0.05). Results Given the seeding density used, we calculated that each SC releases approximately 2.35 × 10 5 ± 3.10 × 10 4 EVs per 24 h. Further, using fluorescent microscopy, we verified SC-EVs deliver cargo into myotubes, some of which was localized to the mitochondria. H 2 O 2 exposure resulted in a 42% decline in peak mitochondrial respiration (p=0.0243) as well as a 46% reduction in spare respiratory capacity (p=0.0185) relative to the untreated control group. Subsequent treatment with SC-EVs (3.12×10 8 SC-EV; 24 h) following H 2 O 2 exposure restored 76% of peak mitochondrial respiration (p=0.0187) and 84% of spare respiratory capacity in the damaged myotubes (p=0.0198). SC-EVs did not affect mitochondrial function in the undamaged myotubes. Conclusion Collectively, these data demonstrate SC-EVs may represent a novel therapeutic approach for treatment of myopathies associated with mitochondrial dysfunction.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":124688,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9505,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":364369,"name":"Brittany E. Wilson","orcid":"0000-0003-4564-8110","position":1,"is_corresponding":false},{"id":364370,"name":"Eric R. Muñoz","orcid":"0000-0003-0010-7513","position":2,"is_corresponding":false},{"id":364371,"name":"Andrew D. Mitchell","orcid":"0000-0002-2018-4359","position":3,"is_corresponding":false},{"id":364372,"name":"Joshua T. Selsby","orcid":"0000-0003-3797-7539","position":4,"is_corresponding":false},{"id":364373,"name":"Matthew B. Hudson","orcid":"0000-0003-0998-0191","position":5,"is_corresponding":false},{"id":364368,"name":"Kyle T. Shuler","orcid":"0000-0001-5948-4714","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-18T23:15:11.632153Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}