{"doi":"10.1101/2020.02.03.929158","title":"Phenotypic variation within and across transcriptomic cell types in mouse motor cortex","abstract":"Cortical neurons exhibit astounding diversity in gene expression as well as in morphological and electrophysiological properties. Most existing neural taxonomies are based on either transcriptomic or morpho-electric criteria, as it has been technically challenging to study both aspects of neuronal diversity in the same set of cells. Here we used Patch-seq to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of over 1300 neurons in adult mouse motor cortex, providing a comprehensive morpho-electric annotation of almost all transcriptomically defined neural cell types. We found that, although broad families of transcriptomic types ( Vip , Pvalb , Sst , etc.) had distinct and essentially non-overlapping morpho-electric phenotypes, individual transcriptomic types within the same family were not well-separated in the morpho-electric space. Instead, there was a continuum of variability in morphology and electrophysiology, with neighbouring transcriptomic cell types showing similar morpho-electric features, often without clear boundaries between them. Our results suggest that neural types in the neocortex do not always form discrete entities. Instead, neurons follow a hierarchy consisting of distinct non-overlapping branches at the level of families, but can form continuous and correlated transcriptomic and morpho-electrical landscapes within families.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":118671,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":48,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9093,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":58949,"name":"Dmitry Kobak","orcid":"0000-0002-5639-7209","position":1,"is_corresponding":false},{"id":59053,"name":"Matteo Bernabucci","orcid":"0000-0003-4458-117X","position":2,"is_corresponding":false},{"id":59054,"name":"Yves Bernaerts","orcid":"0000-0003-4948-0423","position":3,"is_corresponding":false},{"id":59109,"name":"Cathryn R. Cadwell","orcid":"0000-0003-1963-8285","position":4,"is_corresponding":false},{"id":59056,"name":"Jesus Ramon Castro","orcid":"0000-0002-6628-980X","position":5,"is_corresponding":false},{"id":59057,"name":"Leonard Hartmanis","orcid":"0000-0002-4922-8781","position":6,"is_corresponding":false},{"id":229159,"name":"Xiaolong Jiang","orcid":"0000-0001-8066-1383","position":7,"is_corresponding":false},{"id":229160,"name":"Sophie Laturnus","orcid":"0000-0001-9532-788X","position":8,"is_corresponding":false},{"id":59059,"name":"Elanine Miranda","orcid":"0000-0002-1633-9303","position":9,"is_corresponding":false},{"id":229161,"name":"Shalaka Mulherkar","orcid":"0000-0001-8736-527X","position":10,"is_corresponding":false},{"id":59062,"name":"Zheng Huan Tan","orcid":"0000-0002-1886-2421","position":11,"is_corresponding":false},{"id":19354,"name":"Zizhen Yao","orcid":"0000-0002-9361-5607","position":12,"is_corresponding":false},{"id":23581,"name":"Hongkui Zeng","orcid":"0000-0002-0326-5878","position":13,"is_corresponding":false},{"id":229162,"name":"Rickard Sandberg","orcid":"0000-0001-6473-1740","position":14,"is_corresponding":false},{"id":58947,"name":"Philipp Berens","orcid":"0000-0002-0199-4727","position":15,"is_corresponding":false},{"id":59098,"name":"Andreas S. Tolias","orcid":"0000-0002-4305-6376","position":16,"is_corresponding":false},{"id":58952,"name":"Federico Scala","orcid":"0000-0002-2680-8572","position":0,"is_corresponding":true}],"reference_count":76,"raw_metadata":null,"created_at":"2026-07-18T23:14:03.409507Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}