{"doi":"10.1101/2020.02.01.929877","title":"G-baToN: a versatile reporter system for cancer cell-stromal cell interactions","abstract":"ABSTRACT Cell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, interactions between cancer cells and stromal cells play a major role in nearly every step of carcinogenesis. Thus, the ability to record cell-cell interactions would facilitate mechanistic delineation of the role of cancer microenvironment. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon nanobody-directed fluorescent protein transfer to enable sensitive and specific labeling of cells after cell-cell interactions. G-baToN is a generalizable system that enables physical contact-based labeling between various cell types, including diverse cancer-stromal cell pairs. A suite of orthogonal baToN tools enables reciprocal cell-cell labeling, interaction-dependent cargo transfer, and the identification of higher-order cell-cell interactions across a wide range of cell types. The ability to track physically interacting cells with these simple and sensitive systems will greatly accelerate our understanding of the outputs of cell-cell interactions in cancer as well as across many biological processes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":131579,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":354278,"name":"Christopher W. Murray","orcid":"0000-0002-6551-2053","position":1,"is_corresponding":false},{"id":354279,"name":"Ian L. Linde","orcid":"0000-0002-6077-7466","position":2,"is_corresponding":false},{"id":354280,"name":"Nicholas J. Kramer","orcid":"0000-0003-4557-8343","position":3,"is_corresponding":false},{"id":354281,"name":"Zhonglin Lyu","orcid":"0000-0003-3229-8596","position":4,"is_corresponding":false},{"id":354282,"name":"Min K. Tsai","orcid":"0000-0003-4732-4259","position":5,"is_corresponding":false},{"id":585653,"name":"Yi Chen","orcid":"0000-0001-7960-8374","position":6,"is_corresponding":false},{"id":354283,"name":"Hongchen Cai","orcid":"0000-0002-5921-5773","position":7,"is_corresponding":false},{"id":230690,"name":"Aaron D. Gitler","orcid":"0000-0001-8603-1526","position":8,"is_corresponding":false},{"id":14548,"name":"Edgar G. Engleman","orcid":"0000-0002-2096-9279","position":9,"is_corresponding":false},{"id":354284,"name":"Wonjae Lee","orcid":"0000-0003-1524-2259","position":10,"is_corresponding":false},{"id":71713,"name":"Monte M. Winslow","orcid":"0000-0002-5730-9573","position":11,"is_corresponding":false},{"id":354277,"name":"Rui Tang","orcid":"0000-0002-6950-9580","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-18T23:16:03.875886Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}