{"doi":"10.1101/2020.01.31.927327","title":"A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission","abstract":"Abstract The ESCRT complexes drive membrane scission in HIV-1 release, autophagosome closure, MVB biogenesis, cytokinesis, and other cell processes. ESCRT-I is the most upstream complex and bridges the system to HIV-1 Gag in virus release. The crystal structure of the headpiece of human ESCRT-I comprising TSG101:VPS28:VPS37B:MVB12A was determined, revealing an ESCRT-I helical assembly with a 12 molecule repeat. Electron microscopy confirmed that ESCRT-I subcomplexes form helical filaments in solution. Mutation of VPS28 helical interface residues blocks filament formation in vitro and autophagosome closure and HIV-1 release in human cells. Coarse grained simulations of ESCRT assembly at HIV-1 budding sites suggest that formation of a 12-membered ring of ESCRT-I molecules is a geometry-dependent checkpoint during late stages of Gag assembly and HIV-1 budding, and templates ESCRT-III assembly for membrane scission. These data show that ESCRT-I is not merely a bridging adaptor, but has an essential scaffolding and mechanical role in its own right.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":123414,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9511,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":478987,"name":"Yoshinori Takahashi","orcid":"0000-0002-8004-4817","position":1,"is_corresponding":false},{"id":566284,"name":"Arpa Hudait","orcid":"0000-0002-4279-4000","position":2,"is_corresponding":false},{"id":484585,"name":"Kevin Rose","orcid":"0000-0002-9373-4147","position":3,"is_corresponding":false},{"id":566285,"name":"Nicholas Tjahjono","orcid":"0000-0003-0743-3581","position":4,"is_corresponding":false},{"id":246695,"name":"Alexander J. Pak","orcid":"0000-0003-2823-6480","position":5,"is_corresponding":false},{"id":281833,"name":"Adam L. Yokom","orcid":"0000-0002-3746-7961","position":6,"is_corresponding":false},{"id":349937,"name":"Xinwen Liang","orcid":"0009-0002-2319-4912","position":7,"is_corresponding":false},{"id":228901,"name":"Hong‐Gang Wang","orcid":"0000-0003-0551-0571","position":8,"is_corresponding":false},{"id":485623,"name":"Fadila Bouamr","orcid":null,"position":9,"is_corresponding":false},{"id":246697,"name":"Gregory A. Voth","orcid":"0000-0002-3267-6748","position":10,"is_corresponding":false},{"id":237133,"name":"James H. Hurley","orcid":"0000-0001-5054-5445","position":11,"is_corresponding":false},{"id":237128,"name":"Thomas G. Flower","orcid":"0000-0002-7890-6473","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":null,"created_at":"2026-07-18T23:14:59.547352Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}