{"doi":"10.1101/2020.01.27.922112","title":"Injectable therapeutic organoids using sacrificial hydrogels","abstract":"Abstract Organoids, by promoting self-organization of cells into native-like structures, are becoming widespread in drug-screening technologies, but have so far been used sparingly for cell therapy as current approaches for producing self-organized cell clusters lack scalability or reproducibility in size and cellular organization. We introduce a method of using hydrogels as sacrificial scaffolds, which allow cells to form self-organized clusters followed by gentle release, resulting in highly reproducible multicellular structures on a large scale. We demonstrated this strategy for endothelial cells and mesenchymal stem cells to self-organize into blood-vessel units, which were injected into mice using hypodermic needles, and observed in real time to rapidly form perfusing vasculature. As cell therapy transforms into a new class of therapeutic modality, this simple method – by making use of the dynamic nature of hydrogels – could offer high yields of self-organized multicellular aggregates with reproducible sizes and cellular architectures.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":124567,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9474,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":477768,"name":"Priya N. Anandakumaran","orcid":"0000-0001-6201-5804","position":1,"is_corresponding":false},{"id":478390,"name":"Rafael zur Nieden","orcid":null,"position":2,"is_corresponding":false},{"id":478391,"name":"Kahmun Lo","orcid":null,"position":3,"is_corresponding":false},{"id":477769,"name":"Wenjie Luo","orcid":"0000-0002-5412-5373","position":4,"is_corresponding":false},{"id":477770,"name":"Christian Park","orcid":"0000-0002-0172-6650","position":5,"is_corresponding":false},{"id":477771,"name":"Chuqiao Huyan","orcid":"0000-0002-8252-5489","position":6,"is_corresponding":false},{"id":570381,"name":"Qinyuouen Fu","orcid":null,"position":7,"is_corresponding":false},{"id":477773,"name":"Ziwei Song","orcid":"0000-0003-1782-0227","position":8,"is_corresponding":false},{"id":428123,"name":"Rajinder P. Singh‐Moon","orcid":"0000-0001-6098-9322","position":9,"is_corresponding":false},{"id":477774,"name":"Janice Chung","orcid":"0000-0002-2941-0287","position":10,"is_corresponding":false},{"id":477775,"name":"Jennifer Goldenberg","orcid":"0000-0001-7731-353X","position":11,"is_corresponding":false},{"id":478392,"name":"Nirali Sampat","orcid":null,"position":12,"is_corresponding":false},{"id":477776,"name":"Tetsuhiro Harimoto","orcid":"0000-0001-8859-7494","position":13,"is_corresponding":false},{"id":477777,"name":"Danielle Bajakian","orcid":"0000-0002-5492-6883","position":14,"is_corresponding":false},{"id":477778,"name":"Brian M. Gillette","orcid":"0000-0001-8395-7227","position":15,"is_corresponding":false},{"id":363669,"name":"Samuel K. Sia","orcid":"0000-0003-0230-3542","position":16,"is_corresponding":false},{"id":477767,"name":"Ninna S. Rossen","orcid":"0000-0003-0501-8050","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-18T23:15:11.632153Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}