{"doi":"10.1101/2020.01.13.904540","title":"Solid-Phase Peptide Capture and Release for Bulk and Single-Molecule Proteomics","abstract":"Abstract The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement of peptides as well as single-molecule techniques. One limiting factor for some of these methods is the need for multiple chemical derivatizations and highly pure proteins free of contaminants. We demonstrate a solid-phase capture strategy suitable for the proteolysis, purification, and subsequent chemical modification of peptides. We use this resin on an HEK293T cell lysate and perform one-pot proteolysis, capture, and derivatization to generate a cellular proteome that identified over 40,000 bead-bound peptides. We also show that this capture can be reversed in a traceless manner, such that it is amenable for single-molecule proteomics techniques. With this technique, we perform a fluorescent labeling and C-terminal derivatization on a peptide and subject it to fluorosequencing, demonstrating that washing the resin is sufficient to remove excess dyes and other reagents prior to single-molecule protein sequencing.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":126125,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.953,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":369239,"name":"Brendan M. Floyd","orcid":"0000-0001-7412-0492","position":1,"is_corresponding":false},{"id":369240,"name":"Angela M. Bardo","orcid":"0000-0002-6637-4748","position":2,"is_corresponding":false},{"id":369241,"name":"Jagannath Swaminathan","orcid":"0000-0002-5005-7182","position":3,"is_corresponding":false},{"id":240136,"name":"Edward M. Marcotte","orcid":"0000-0001-8808-180X","position":4,"is_corresponding":false},{"id":301811,"name":"Eric V. Anslyn","orcid":"0000-0002-5137-8797","position":5,"is_corresponding":false},{"id":369238,"name":"Cecil J. Howard","orcid":"0000-0002-5761-9424","position":0,"is_corresponding":true}],"reference_count":23,"raw_metadata":null,"created_at":"2026-07-18T23:15:23.509897Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}