{"doi":"10.1101/2020.01.10.902478","title":"Reconstruction of motor control circuits in adult <i>Drosophila</i> using automated transmission electron microscopy","abstract":"SUMMARY Many animals use coordinated limb movements to interact with and navigate through the environment. To investigate circuit mechanisms underlying locomotor behavior, we used serial-section electron microscopy (EM) to map synaptic connectivity within a neuronal network that controls limb movements. We present a synapse-resolution EM dataset containing the ventral nerve cord (VNC) of an adult female Drosophila melanogaster . To generate this dataset, we developed GridTape, a technology that combines automated serial-section collection with automated high-throughput transmission EM. Using this dataset, we reconstructed 507 motor neurons, including all those that control the legs and wings. We show that a specific class of leg sensory neurons directly synapse onto the largest-caliber motor neuron axons on both sides of the body, representing a unique feedback pathway for fast limb control. We provide open access to the dataset and reconstructions registered to a standard atlas to permit matching of cells between EM and light microscopy data. We also provide GridTape instrumentation designs and software to make large-scale EM data acquisition more accessible and affordable to the scientific community.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":118905,"datarank":1.0742341105980968,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.5367062698296803,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.5367062698296803,"corpus_percentile":81.13251334416339,"corpus_rank":2440,"citation_count":35,"citer_count":23,"citers_with_citation_signal":19,"citers_with_endowment":19,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.7205,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":552381,"name":"David G. C. Hildebrand","orcid":"0000-0002-2079-3517","position":1,"is_corresponding":false},{"id":258633,"name":"Brett J. Graham","orcid":"0000-0001-6315-4507","position":2,"is_corresponding":false},{"id":552382,"name":"Aaron T. Kuan","orcid":"0000-0002-1908-7949","position":3,"is_corresponding":false},{"id":552383,"name":"Logan A. Thomas","orcid":"0000-0002-7806-3496","position":4,"is_corresponding":false},{"id":552384,"name":"Tri Nguyen","orcid":"0000-0001-9936-1794","position":5,"is_corresponding":false},{"id":553244,"name":"Julia Buhmann","orcid":null,"position":6,"is_corresponding":false},{"id":285973,"name":"Anthony W. Azevedo","orcid":"0000-0001-8318-9678","position":7,"is_corresponding":false},{"id":552385,"name":"Brendan L. Shanny","orcid":"0000-0003-3379-3396","position":8,"is_corresponding":false},{"id":552386,"name":"Jan Funke","orcid":"0000-0003-4388-7783","position":9,"is_corresponding":false},{"id":285978,"name":"John C Tuthill","orcid":"0000-0002-5689-5806","position":10,"is_corresponding":false},{"id":256210,"name":"Wei-Chung Allen Lee","orcid":"0000-0002-4618-295X","position":11,"is_corresponding":false},{"id":553243,"name":"Jasper T. Maniates-Selvin","orcid":null,"position":0,"is_corresponding":true}],"reference_count":98,"raw_metadata":null,"created_at":"2026-07-18T23:14:03.409507Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}