{"doi":"10.1101/2020.01.02.891770","title":"Non-canonical glutamate signaling in a genetic model of migraine with aura","abstract":"Summary Migraine with aura is an extremely common but poorly understood sensory circuit disorder. Monogenic models allow an opportunity to understand its mechanisms, in particular because the migraine aura is associated with spreading depolarizations that can be measured physiologically. Using fluorescent glutamate imaging in awake mice carrying a familial hemiplegic migraine type 2 mutation, we recorded previously undescribed spontaneous ‘plumes’ of glutamate signaling that anatomically overlapped with reduced density of GLT-1a positive astrocyte processes. These events could be mimicked in wild-type animals by inhibition of glutamate clearance, which we show to be slower during sensory processing in FHM2 carriers. Plumes depended on calcium mediated vesicular release from neurons, but not action potentials. Importantly, a rise in both basal glutamate and plume frequency predicted the onset of spreading depolarization in WT and FHM2 animals, providing a novel mechanism in migraine with aura and by extension the many other neurological disorders where spreading depolarizations occur.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2020,"id":120625,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9551,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":303919,"name":"Pratyush Suryavanshi","orcid":"0000-0001-6904-7294","position":1,"is_corresponding":false},{"id":303920,"name":"Marcello Melone","orcid":"0000-0003-4173-0774","position":2,"is_corresponding":false},{"id":306121,"name":"Katelyn M. Reinhart","orcid":null,"position":3,"is_corresponding":false},{"id":558630,"name":"Punam Sawant-Pokam","orcid":"0000-0002-3443-868X","position":4,"is_corresponding":false},{"id":303921,"name":"Dan Kaufmann","orcid":"0000-0001-9836-1370","position":5,"is_corresponding":false},{"id":306122,"name":"Jeremy Theriot","orcid":null,"position":6,"is_corresponding":false},{"id":303922,"name":"Arianna Pugliese","orcid":"0000-0001-5132-7642","position":7,"is_corresponding":false},{"id":212680,"name":"Giorgio Carmignoto","orcid":"0000-0001-5853-1566","position":8,"is_corresponding":false},{"id":303923,"name":"C. William Shuttleworth","orcid":"0000-0002-0237-4888","position":9,"is_corresponding":false},{"id":303924,"name":"Daniela Pietrobon","orcid":"0000-0002-5148-8670","position":10,"is_corresponding":false},{"id":303925,"name":"K. C. Brennan","orcid":"0000-0003-4193-5841","position":11,"is_corresponding":false},{"id":303918,"name":"Patrick D. Parker","orcid":"0000-0001-5217-107X","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-18T23:14:38.147936Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}