{"doi":"10.1101/142711","title":"A sequencer coming of age:\n                  <i>de novo</i>\n                  genome assembly using MinION reads","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Nanopore technology provides a novel approach to DNA sequencing that yields long, label-free reads of constant quality. The first commercial implementation of this approach, the MinION, has shown promise in various sequencing applications. The presented literature review gives an up-to-date overview of the MinION’s utility as a\n                  <jats:italic>de novo</jats:italic>\n                  sequencing device. It is argued that the MinION may allow for portable and affordable\n                  <jats:italic>de novo</jats:italic>\n                  sequencing of even complex genomes in the near future, despite the currently error-prone nature of its reads. Through continuous updates to the MinION hardware and the development of new assembly pipelines, both sequencing accuracy and assembly quality have already risen rapidly. However, this fast pace of development has also lead to a lack of oversight in the expanding landscape of analysis tools, as performance evaluations are outdated quickly. Now that the MinION is approaching a state of maturity, a thorough comparative benchmarking effort of\n                  <jats:italic>de novo</jats:italic>\n                  assembly pipelines may be at place.\n                </jats:p>","journal":null,"year":null,"id":687775,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":813405,"name":"Dick de Ridder","orcid":"0000-0002-4944-4310","position":1,"is_corresponding":false},{"id":1796765,"name":"Judith Risse","orcid":null,"position":2,"is_corresponding":false},{"id":813403,"name":"Carlos de Lannoy","orcid":"0000-0002-3754-0508","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A sequencer coming of age:\n                  <i>de novo</i>\n                  genome assembly using MinION reads","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Nanopore technology provides a novel approach to DNA sequencing that yields long, label-free reads of constant quality. The first commercial implementation of this approach, the MinION, has shown promise in various sequencing applications. The presented literature review gives an up-to-date overview of the MinION’s utility as a\n                  <jats:italic>de novo</jats:italic>\n                  sequencing device. It is argued that the MinION may allow for portable and affordable\n                  <jats:italic>de novo</jats:italic>\n                  sequencing of even complex genomes in the near future, despite the currently error-prone nature of its reads. Through continuous updates to the MinION hardware and the development of new assembly pipelines, both sequencing accuracy and assembly quality have already risen rapidly. However, this fast pace of development has also lead to a lack of oversight in the expanding landscape of analysis tools, as performance evaluations are outdated quickly. Now that the MinION is approaching a state of maturity, a thorough comparative benchmarking effort of\n                  <jats:italic>de novo</jats:italic>\n                  assembly pipelines may be at place.\n                </jats:p>","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W2617301133","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2018,"count":2},{"year":2019,"count":1},{"year":2021,"count":1}],"oa_status":"green","license":"cc-by","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2017/05/26/142711.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2017/05/26/142711.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/142711","host_type":"publisher"},{"url":"https://doi.org/10.1101/142711","host_type":"repository"}],"fields_of_study":["Genomics and Phylogenetic Studies","Advanced biosensing and bioanalysis techniques","RNA and protein synthesis mechanisms"],"mesh_terms":[],"keywords":["Minion","Nanopore sequencing","Sequence assembly","Computer science","DNA sequencing","Benchmarking","Computational biology","Hybrid genome assembly","Biology","Reference genome","Genetics","DNA","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Industry, innovation and infrastructure"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T22:41:50.530961Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}