{"doi":"10.1101/048603","title":"Third-generation sequencing and the future of genomics","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Third-generation long-range DNA sequencing and mapping technologies are creating a renaissance in high-quality genome sequencing. Unlike second-generation sequencing, which produces short reads a few hundred base-pairs long, third-generation single-molecule technologies generate over 10,000 bp reads or map over 100,000 bp molecules. We analyze how increased read lengths can be used to address longstanding problems in\n                  <jats:italic>de novo</jats:italic>\n                  genome assembly, structural variation analysis and haplotype phasing.\n                </jats:p>","journal":null,"year":null,"id":607206,"datarank":0.7465100613630863,"base_score":4.976733742420574,"endowment":4.976733742420574,"self_citation_contribution":0.7465100613630863,"citation_network_contribution":0.0,"self_endowment_contribution":0.7465100613630863,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":144,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1559079,"name":"James Gurtowski","orcid":null,"position":1,"is_corresponding":false},{"id":87350,"name":"Shinjae Yoo","orcid":"0000-0003-4378-6448","position":2,"is_corresponding":false},{"id":30897,"name":"Maria Nattestad","orcid":"0000-0002-4796-2894","position":3,"is_corresponding":false},{"id":1559081,"name":"Shoshana Marcus","orcid":null,"position":4,"is_corresponding":false},{"id":107894,"name":"Sara Goodwin","orcid":"0000-0002-6110-7296","position":5,"is_corresponding":false},{"id":33868,"name":"W. Richard McCombie","orcid":"0000-0003-1899-0682","position":6,"is_corresponding":false},{"id":24539,"name":"Michael C. Schatz","orcid":"0000-0002-4118-4446","position":7,"is_corresponding":false},{"id":255341,"name":"Hayan Lee","orcid":"0000-0003-0571-3192","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Third-generation sequencing and the future of genomics","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Third-generation long-range DNA sequencing and mapping technologies are creating a renaissance in high-quality genome sequencing. Unlike second-generation sequencing, which produces short reads a few hundred base-pairs long, third-generation single-molecule technologies generate over 10,000 bp reads or map over 100,000 bp molecules. We analyze how increased read lengths can be used to address longstanding problems in\n                  <jats:italic>de novo</jats:italic>\n                  genome assembly, structural variation analysis and haplotype phasing.\n                </jats:p>","is_dataset_classified":null,"base_score":4.976733742420574,"endowment":4.976733742420574,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23304386","pmcid":null,"openalex_id":"https://openalex.org/W2337513885","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2016,"count":4},{"year":2017,"count":21},{"year":2018,"count":20},{"year":2019,"count":25},{"year":2020,"count":9},{"year":2021,"count":16},{"year":2022,"count":19},{"year":2023,"count":9},{"year":2024,"count":8},{"year":2025,"count":6},{"year":2026,"count":6}],"oa_status":"green","license":"cc-by-nc","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2016/04/13/048603.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2016/04/13/048603.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/048603","host_type":"publisher"},{"url":"https://doi.org/10.1101/048603","host_type":"repository"}],"fields_of_study":["Genomics and Phylogenetic Studies","RNA and protein synthesis mechanisms","Genomics and Rare Diseases"],"mesh_terms":[],"keywords":["DNA sequencing","Structural variation","Hybrid genome assembly","Genomics","Computational biology","Sequence assembly","Genome","Biology","Haplotype","Third generation","Genetics","DNA","Computer science","Gene","Allele"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T05:58:00.683338Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}