{"doi":"10.1099/vir.0.80481-0","title":"Enhanced immunogenicity using an alphavirus replicon DNA vaccine against human immunodeficiency virus type 1","abstract":"<jats:p>With the human immunodeficiency virus type 1 (HIV-1) epidemic expanding at increasing speed, development of a safe and effective vaccine remains a high priority. One of the most central vaccine platforms considered is plasmid DNA. However, high doses of DNA and several immunizations are typically needed to achieve detectable T-cell responses. In this study, a Semliki Forest virus replicon DNA vaccine designed for human clinical trials, DREP.HIVA, encoding an antigen that is currently being used in human trials in the context of a conventional DNA plasmid, pTHr.HIVA, was generated. It was shown that a single immunization of DREP.HIVA stimulated HIV-1-specific T-cell responses in mice, suggesting that the poor immunogenicity of conventional DNA vaccines may be enhanced by using viral replicon-based plasmid systems. The results presented here support the evaluation of Semliki Forest virus replicon DNA vaccines in non-human primates and in clinical studies.</jats:p>","journal":"Journal of General Virology","year":2005,"id":16653,"datarank":2.0601645336993313,"base_score":3.828641396489095,"endowment":3.828641396489095,"self_citation_contribution":0.5742962094733643,"citation_network_contribution":1.485868324225967,"self_endowment_contribution":0.5742962094733643,"citer_contribution":1.485868324225967,"corpus_percentile":null,"corpus_rank":null,"citation_count":45,"citer_count":31,"citers_with_citation_signal":28,"citers_with_endowment":28,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":122134,"name":"Mattias N. E. Forsell","orcid":null,"position":1,"is_corresponding":false},{"id":122135,"name":"Christina Barnfield","orcid":null,"position":2,"is_corresponding":false},{"id":122136,"name":"Eivor Bonin","orcid":null,"position":3,"is_corresponding":false},{"id":122137,"name":"Tomas Hanke","orcid":null,"position":4,"is_corresponding":false},{"id":122138,"name":"Magnus Sundström","orcid":null,"position":5,"is_corresponding":false},{"id":122139,"name":"Gunilla B. Karlsson","orcid":null,"position":6,"is_corresponding":false},{"id":122140,"name":"Peter Liljeström","orcid":null,"position":7,"is_corresponding":false},{"id":122133,"name":"Eva K. L. Nordström","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.828641396489095,"endowment":3.828641396489095,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15659754","pmcid":null,"openalex_id":"https://openalex.org/W1982057259","authors":[],"funders":[],"total_grants":0,"fwci":1.2377,"citation_percentile":0.74778761,"influential_citations":2,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":3},{"year":2014,"count":6},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2018,"count":3},{"year":2019,"count":1},{"year":2024,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://doi.org/10.1099/vir.0.80481-0","host_type":"BRONZE"},{"url":"https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80481-0?crawler=true","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15659754","host_type":"repository"},{"url":"https://ora.ox.ac.uk/objects/uuid:8399b41c-00c0-4585-802c-7ccb18b01434","host_type":"repository"}],"fields_of_study":["HIV Research and Treatment","vaccines and immunoinformatics approaches","Mosquito-borne diseases and control","Biology","Medicine","Animals","DNA, Viral","Drug Evaluation, Preclinical","Female","HIV Infections","HIV-1","Mice","Mice, Inbred BALB C","Replicon","Semliki forest virus","T-Lymphocytes","Vaccination","Vaccines, DNA","Viral Vaccines"],"mesh_terms":["Animals","DNA, Viral","Drug Evaluation, Preclinical","Female","Mice, Inbred BALB C","Replicon","Semliki forest virus","T-Lymphocytes","Vaccination","Viral Vaccines","HIV-1","HIV Infections","Vaccines, DNA","Mice"],"keywords":["Replicon","Semliki Forest virus","Virology","Immunogenicity","Biology","DNA vaccination","Alphavirus","Plasmid","Virus","Context (archaeology)","AIDS Vaccines","DNA","Antigen","Immunology","Vaccine trial","Vaccination","RNA","Genetics","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T12:21:25.451529Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}