{"doi":"10.1099/vir.0.058008-0","title":"Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon","abstract":"<jats:p>A method for rapid production of single-round infectious particles (SRIPs) of flavivirus would be useful for viral mutagenesis studies. Here, we established a DNA-based production system for SRIPs of flavivirus. We constructed a Japanese encephalitis virus (JEV) subgenomic replicon plasmid, which lacked the C-prM-E (capsid–pre-membrane–envelope) coding region, under the control of the cytomegalovirus promoter. When the JEV replicon plasmid was transiently co-transfected with a JEV C-prM-E expression plasmid into 293T cells, SRIPs were produced, indicating successful <jats:italic>trans</jats:italic>-complementation with JEV structural proteins. Equivalent production levels were observed when C and prM–E proteins were provided separately. Furthermore, dengue types 1–4, West Nile, yellow fever or tick-borne encephalitis virus prM-E proteins could be utilized for production of chimaeric flavivirus SRIPs, although the production was less efficient for dengue and yellow fever viruses. These results indicated that our plasmid-based system is suitable for investigating the life cycles of flaviviruses, diagnostic applications and development of safer vaccine candidates.</jats:p>","journal":"Journal of General Virology","year":2014,"id":32039,"datarank":1.3046353199716538,"base_score":3.8501476017100584,"endowment":3.8501476017100584,"self_citation_contribution":0.5775221402565088,"citation_network_contribution":0.7271131797151449,"self_endowment_contribution":0.5775221402565088,"citer_contribution":0.7271131797151449,"corpus_percentile":null,"corpus_rank":null,"citation_count":46,"citer_count":25,"citers_with_citation_signal":19,"citers_with_endowment":19,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":170430,"name":"Tomohiro Ishikawa","orcid":null,"position":1,"is_corresponding":false},{"id":136081,"name":"Eiji Konishi","orcid":null,"position":2,"is_corresponding":false},{"id":151019,"name":"Mami Matsuda","orcid":null,"position":3,"is_corresponding":false},{"id":170431,"name":"Koichi Watashi","orcid":null,"position":4,"is_corresponding":false},{"id":139797,"name":"Hideki Aizaki","orcid":null,"position":5,"is_corresponding":false},{"id":138175,"name":"Tomohiko Takasaki","orcid":null,"position":6,"is_corresponding":false},{"id":150698,"name":"Takaji Wakita","orcid":null,"position":7,"is_corresponding":false},{"id":150731,"name":"Ryosuke Suzuki","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.8501476017100584,"endowment":3.8501476017100584,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24096319","pmcid":null,"openalex_id":"https://openalex.org/W2124634488","authors":[],"funders":[],"total_grants":0,"fwci":3.3264,"citation_percentile":0.91791703,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":4},{"year":2015,"count":2},{"year":2016,"count":3},{"year":2017,"count":8},{"year":2018,"count":4},{"year":2019,"count":3},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2023,"count":3},{"year":2024,"count":4},{"year":2025,"count":5},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://doi.org/10.1099/vir.0.058008-0","host_type":"BRONZE"},{"url":"https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.058008-0?crawler=true","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24096319","host_type":"repository"}],"fields_of_study":["Mosquito-borne diseases and control","Insect symbiosis and bacterial influences","Malaria Research and Control","Biology","Medicine","Cell Line","Chimera","Defective Viruses","Encephalitis Virus, Japanese","Flavivirus","Humans","Plasmids","Replicon","Virus Replication"],"mesh_terms":["Cell Line","Chimera","Defective Viruses","Encephalitis Virus, Japanese","Flavivirus","Humans","Plasmids","Replicon","Virus Replication"],"keywords":["Replicon","Virology","Flavivirus","Biology","Plasmid","Japanese encephalitis","Dengue fever","Subgenomic mRNA","Virus","Dengue virus","Capsid","Encephalitis","DNA","CRISPR","Gene","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Responsible consumption and production"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T10:02:22.035876Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}